7cu8

Crystal structure of the soluble domain of TiME protein from Mycobacterium tuberculosis

spinqualitylabelsall models 7cu8, resolution 3.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7cu8 is a 7 chain structure with sequence from Mycobacterium tuberculosis H37Rv. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.3Å Ligands: SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function I6XI06_MYCTU Publication Abstract from PubMed Tuberculosis-causing mycobacteria have thick cell-wall and capsule layers that are formed from complex structures. Protein secretion across these barriers depends on a specialized protein secretion system, but none has been reported. We show that Mycobacterium tuberculosis Rv3705c and its homologous MSMEG_6251 in Mycobacterium smegmatis are tube-forming proteins in the mycobacterial envelope (TiME). Crystallographic and cryo-EM structures of these two proteins show that both proteins form rotationally symmetric rings. Two layers of TiME rings pack together in a tail-to-tail manner into a ring-shaped complex, which, in turn, stacks together to form tubes. M. smegmatis TiME was detected mainly in the cell wall and capsule. Knocking out the TiME gene markedly decreased the amount of secreted protein in the M. smegmatis culture medium, and expression of this gene in knocked-out strain partially restored the level of secreted protein. Our structure and functional data thus suggest that TiME forms a protein transport tube across the mycobacterial outer envelope. Identification and architecture of a putative secretion tube across mycobacterial outer envelope.,Cai X, Liu L, Qiu C, Wen C, He Y, Cui Y, Li S, Zhang X, Zhang L, Tian C, Bi L, Zhou ZH, Gong W Sci Adv. 2021 Aug 20;7(34). pii: 7/34/eabg5656. doi: 10.1126/sciadv.abg5656., Print 2021 Aug. PMID:34417177 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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