7xs4: Difference between revisions

Latest revision as of 17:59, 29 November 2023

Crystal structure of URT1 in complex with AAAU RNA

Structural highlights

7xs4 is a 2 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.846Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

URT1_ARATH UTP:RNA uridylyltransferase with a marked preference for uridine polymerization and a distributive activity for the first added nucleotides (PubMed:23748567, PubMed:25928405). Uridylates oligo(A)-tailed mRNAs to prevent 3' to 5' ribonucleotytic attacks (PubMed:23748567). Can prevent the 3' trimming of mRNAs still engaged on polysomes (PubMed:23748567). Acts synergistically with HESO1 in unmethylated miRNA uridylation, leading to their degradation (PubMed:25928341). URT1 and HESO1 prefer substrates with different 3' end nucleotides and act cooperatively to tail different forms of the same miRNAs (PubMed:25928405). URT1 and HESO1 act sequentially, with URT1 mono-uridylating the miRNAs followed by their further uridylation by HESO1 (PubMed:25928405). Has no effect on uridylation of heterochromatic siRNAs (PubMed:25928341). Able to act on AGO1-bound miRNAs and the uridylated species stay associated with AGO1 (PubMed:25928405).^[1] ^[2] ^[3]

References

↑ Sement FM, Ferrier E, Zuber H, Merret R, Alioua M, Deragon JM, Bousquet-Antonelli C, Lange H, Gagliardi D. Uridylation prevents 3' trimming of oligoadenylated mRNAs. Nucleic Acids Res. 2013 Aug;41(14):7115-27. doi: 10.1093/nar/gkt465. Epub 2013, Jun 6. PMID:23748567 doi:https://dx.doi.org/10.1093/nar/gkt465
↑ Wang X, Zhang S, Dou Y, Zhang C, Chen X, Yu B, Ren G. Synergistic and independent actions of multiple terminal nucleotidyl transferases in the 3' tailing of small RNAs in Arabidopsis. PLoS Genet. 2015 Apr 30;11(4):e1005091. doi: 10.1371/journal.pgen.1005091., eCollection 2015 Apr. PMID:25928341 doi:https://dx.doi.org/10.1371/journal.pgen.1005091
↑ Tu B, Liu L, Xu C, Zhai J, Li S, Lopez MA, Zhao Y, Yu Y, Ramachandran V, Ren G, Yu B, Li S, Meyers BC, Mo B, Chen X. Distinct and cooperative activities of HESO1 and URT1 nucleotidyl transferases in microRNA turnover in Arabidopsis. PLoS Genet. 2015 Apr 30;11(4):e1005119. doi: 10.1371/journal.pgen.1005119., eCollection 2015 Apr. PMID:25928405 doi:https://dx.doi.org/10.1371/journal.pgen.1005119

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Sement FM, Ferrier E, Zuber H, Merret R, Alioua M, Deragon JM, Bousquet-Antonelli C, Lange H, Gagliardi D. Uridylation prevents 3' trimming of oligoadenylated mRNAs. Nucleic Acids Res. 2013 Aug;41(14):7115-27. doi: 10.1093/nar/gkt465. Epub 2013, Jun 6. PMID:23748567 doi:https://dx.doi.org/10.1093/nar/gkt465

[2] Wang X, Zhang S, Dou Y, Zhang C, Chen X, Yu B, Ren G. Synergistic and independent actions of multiple terminal nucleotidyl transferases in the 3' tailing of small RNAs in Arabidopsis. PLoS Genet. 2015 Apr 30;11(4):e1005091. doi: 10.1371/journal.pgen.1005091., eCollection 2015 Apr. PMID:25928341 doi:https://dx.doi.org/10.1371/journal.pgen.1005091

[3] Tu B, Liu L, Xu C, Zhai J, Li S, Lopez MA, Zhao Y, Yu Y, Ramachandran V, Ren G, Yu B, Li S, Meyers BC, Mo B, Chen X. Distinct and cooperative activities of HESO1 and URT1 nucleotidyl transferases in microRNA turnover in Arabidopsis. PLoS Genet. 2015 Apr 30;11(4):e1005119. doi: 10.1371/journal.pgen.1005119., eCollection 2015 Apr. PMID:25928405 doi:https://dx.doi.org/10.1371/journal.pgen.1005119

[1]

[2]

[3]

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[7xs4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XS4 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xs4 OCA], [https://pdbe.org/7xs4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xs4 RCSB], [https://www.ebi.ac.uk/pdbsum/7xs4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xs4 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.846&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xs4 OCA], [https://pdbe.org/7xs4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xs4 RCSB], [https://www.ebi.ac.uk/pdbsum/7xs4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xs4 ProSAT]</span></td></tr>
 </table>
 == Function ==

7xs4: Difference between revisions

Latest revision as of 17:59, 29 November 2023

Crystal structure of URT1 in complex with AAAU RNA

Structural highlights

Function

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools