7y7s

QDE-1 in complex with DNA template, RNA primer and AMPNPP

spinqualitylabelsall models 7y7s, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7y7s is a 6 chain structure with sequence from Neurospora crassa and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.7Å Ligands: CA, MG, PEG, ZAN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q9Y7G6_NEUCS Publication Abstract from PubMed Neurospora crassa protein QDE-1, a member of the two-barrel polymerase superfamily, possesses both DNA- and RNA-dependent RNA polymerase (DdRP and RdRP) activities. The dual activities are essential for the production of double-stranded RNAs (dsRNAs), the precursors of small interfering RNAs (siRNAs) in N. crassa. Here, we report five complex structures of N-terminal truncated QDE-1 (QDE-1DeltaN), representing four different reaction states: DNA/RNA-templated elongation, the de novo initiation of RNA synthesis, the first step of nucleotide condensation during de novo initiation and initial NTP loading. The template strand is aligned by a bridge-helix and double-psi beta-barrels 2 (DPBB2), the RNA product is held by DPBB1 and the slab domain. The DNA template unpairs with the RNA product at position -7, but the RNA template remains paired. The NTP analog coordinates with cations and is precisely positioned at the addition site by a rigid trigger loop and a proline-containing loop in the active center. The unique C-terminal tail from the QDE-1 dimer partner inserts into the substrate-binding cleft and plays regulatory roles in RNA synthesis. Collectively, this work elucidates the conserved mechanisms for DNA/RNA-dependent dual activities by QDE-1 and other two-barrel polymerase superfamily members. Structural insights into the dual activities of the two-barrel RNA polymerase QDE-1.,Cui R, Li H, Zhao J, Li X, Gan J, Ma J Nucleic Acids Res. 2022 Sep 23;50(17):10169-10186. doi: 10.1093/nar/gkac727. PMID:36039765 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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