8u0t: Difference between revisions

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'''Unreleased structure'''


The entry 8u0t is ON HOLD  until Paper Publication
==PRD-0038 RBD bound to Rhinolophus alcyone ACE2 (local refinement)==
<StructureSection load='8u0t' size='340' side='right'caption='[[8u0t]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8u0t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhinolophus_alcyone Rhinolophus alcyone] and [https://en.wikipedia.org/wiki/Sarbecovirus Sarbecovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8U0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8U0T FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8u0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8u0t OCA], [https://pdbe.org/8u0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8u0t RCSB], [https://www.ebi.ac.uk/pdbsum/8u0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8u0t ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0N7IQX6_RHIAY A0A0N7IQX6_RHIAY]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Although Rhinolophus bats harbor diverse clade 3 sarbecoviruses, the structural determinants of receptor tropism along with the antigenicity of their spike (S) glycoproteins remain uncharacterized. Here, we show that the African Rhinolophus bat clade 3 sarbecovirus PRD-0038 S has a broad angiotensin-converting enzyme 2 (ACE2) usage and that receptor-binding domain (RBD) mutations further expand receptor promiscuity and enable human ACE2 utilization. We determine a cryo-EM structure of the PRD-0038 RBD bound to Rhinolophusalcyone ACE2, explaining receptor tropism and highlighting differences with SARS-CoV-1 and SARS-CoV-2. Characterization of PRD-0038 S using cryo-EM and monoclonal antibody reactivity reveals its distinct antigenicity relative to SARS-CoV-2 and identifies PRD-0038 cross-neutralizing antibodies for pandemic preparedness. PRD-0038 S vaccination elicits greater titers of antibodies cross-reacting with vaccine-mismatched clade 2 and clade 1a sarbecoviruses compared with SARS-CoV-2 S due to broader antigenic targeting, motivating the inclusion of clade 3 antigens in next-generation vaccines for enhanced resilience to viral evolution.


Authors:  
Broad receptor tropism and immunogenicity of a clade 3 sarbecovirus.,Lee J, Zepeda SK, Park YJ, Taylor AL, Quispe J, Stewart C, Leaf EM, Treichel C, Corti D, King NP, Starr TN, Veesler D Cell Host Microbe. 2023 Nov 16:S1931-3128(23)00422-5. doi: , 10.1016/j.chom.2023.10.018. PMID:37989312<ref>PMID:37989312</ref>


Description:  
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 8u0t" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rhinolophus alcyone]]
[[Category: Sarbecovirus]]
[[Category: Park YJ]]
[[Category: Veesler D]]
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