1ryj: Difference between revisions

Latest revision as of 09:22, 6 December 2023

Solution NMR Structure of Protein Mth1743 from Methanobacterium thermoautotrophicum. Ontario Centre for Structural Proteomics target MTH1743_1_70; Northeast Structural Genomics Consortium Target TT526.

Structural highlights

1ryj is a 1 chain structure with sequence from Methanothermococcus thermolithotrophicus. This structure supersedes the now removed PDB entry 1jsb. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

O27775_METTH

Publication Abstract from PubMed

The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.

An NMR approach to structural proteomics.,Yee A, Chang X, Pineda-Lucena A, Wu B, Semesi A, Le B, Ramelot T, Lee GM, Bhattacharyya S, Gutierrez P, Denisov A, Lee CH, Cort JR, Kozlov G, Liao J, Finak G, Chen L, Wishart D, Lee W, McIntosh LP, Gehring K, Kennedy MA, Edwards AM, Arrowsmith CH Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1825-30. PMID:11854485^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yee A, Chang X, Pineda-Lucena A, Wu B, Semesi A, Le B, Ramelot T, Lee GM, Bhattacharyya S, Gutierrez P, Denisov A, Lee CH, Cort JR, Kozlov G, Liao J, Finak G, Chen L, Wishart D, Lee W, McIntosh LP, Gehring K, Kennedy MA, Edwards AM, Arrowsmith CH. An NMR approach to structural proteomics. Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1825-30. PMID:11854485 doi:10.1073/pnas.042684599

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Yee A, Chang X, Pineda-Lucena A, Wu B, Semesi A, Le B, Ramelot T, Lee GM, Bhattacharyya S, Gutierrez P, Denisov A, Lee CH, Cort JR, Kozlov G, Liao J, Finak G, Chen L, Wishart D, Lee W, McIntosh LP, Gehring K, Kennedy MA, Edwards AM, Arrowsmith CH. An NMR approach to structural proteomics. Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1825-30. PMID:11854485 doi:10.1073/pnas.042684599

[1]

@@ Line 1: / Line 1: @@
 ==Solution NMR Structure of Protein Mth1743 from Methanobacterium thermoautotrophicum. Ontario Centre for Structural Proteomics target MTH1743_1_70; Northeast Structural Genomics Consortium Target TT526.==
-<StructureSection load='1ryj' size='340' side='right'caption='[[1ryj]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='1ryj' size='340' side='right'caption='[[1ryj]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1ryj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_35097 Atcc 35097]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1jsb 1jsb]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RYJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RYJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1ryj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermococcus_thermolithotrophicus Methanothermococcus thermolithotrophicus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1jsb 1jsb]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RYJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RYJ FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">archaea ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2186 ATCC 35097])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ryj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ryj OCA], [https://pdbe.org/1ryj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ryj RCSB], [https://www.ebi.ac.uk/pdbsum/1ryj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ryj ProSAT], [https://www.topsan.org/Proteins/NESGC/1ryj TOPSAN]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/O27775_METTH O27775_METTH]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 35097]]
 [[Category: Large Structures]]
-[[Category: Arrowsmith, C H]]
+[[Category: Methanothermococcus thermolithotrophicus]]
-[[Category: Bhattacharyya, S]]
+[[Category: Arrowsmith CH]]
-[[Category: Chang, X]]
+[[Category: Bhattacharyya S]]
-[[Category: Chen, L]]
+[[Category: Chang X]]
-[[Category: Cort, J R]]
+[[Category: Chen L]]
-[[Category: Denisov, A]]
+[[Category: Cort JR]]
-[[Category: Edwards, A M]]
+[[Category: Denisov A]]
-[[Category: Finak, G]]
+[[Category: Edwards AM]]
-[[Category: Gehring, K]]
+[[Category: Finak G]]
-[[Category: Gutierrez, P]]
+[[Category: Gehring K]]
-[[Category: Kennedy, M A]]
+[[Category: Gutierrez P]]
-[[Category: Kozlov, G]]
+[[Category: Kennedy MA]]
-[[Category: Le, B]]
+[[Category: Kozlov G]]
-[[Category: Lee, C H]]
+[[Category: Le B]]
-[[Category: Lee, G M]]
+[[Category: Lee CH]]
-[[Category: Lee, W]]
+[[Category: Lee GM]]
-[[Category: Liao, J]]
+[[Category: Lee W]]
-[[Category: McIntosh, L P]]
+[[Category: Liao J]]
-[[Category: Structural genomic]]
+[[Category: McIntosh LP]]
-[[Category: Pineda-Lucena, A]]
+[[Category: Pineda-Lucena A]]
-[[Category: Ramelot, T]]
+[[Category: Ramelot T]]
-[[Category: Semesi, A]]
+[[Category: Semesi A]]
-[[Category: Wishart, D]]
+[[Category: Wishart D]]
-[[Category: Wu, B]]
+[[Category: Wu B]]
-[[Category: Yee, A]]
+[[Category: Yee A]]
-[[Category: Beta/alpha protein]]
-[[Category: Nesg]]
-[[Category: Ocsp]]
-[[Category: PSI, Protein structure initiative]]
-[[Category: Unknown function]]

1ryj: Difference between revisions

Latest revision as of 09:22, 6 December 2023

Solution NMR Structure of Protein Mth1743 from Methanobacterium thermoautotrophicum. Ontario Centre for Structural Proteomics target MTH1743_1_70; Northeast Structural Genomics Consortium Target TT526.

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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