2cj4: Difference between revisions

Revision as of 14:19, 13 December 2023

Crystal Structure of a Cell Wall Invertase Inhibitor from Tobacco at pH 4.6

Structural highlights

2cj4 is a 2 chain structure with sequence from Nicotiana tabacum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.63Å
Ligands:	ACT, SO4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O49908_TOBAC

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Plant acid invertases catalyse the breakdown of sucrose. Their activity is tightly regulated through interaction with specific protein inhibitors. The complex between the cell-wall invertase inhibitor Nt-CIF and its target enzyme is stable only at acidic pH, as found in the plant cell wall. Since the pH in this compartment can be modulated between pH 4 and 6 in planta, the rapid dissociation of the inhibitor-enzyme complex at neutral pH may represent a regulatory event. Here, it is analyzed whether the inhibitory component undergoes structural rearrangements upon changes in the pH environment. Six crystal forms grown at pH 4.6-9.5 and diffracting up to 1.63 Angstrom indicate only small structural changes in CIF. This suggests that complex dissociation at neutral pH is mediated either by rearrangements in the enzyme or by a complex pattern of surface charges in the inhibitor-enzyme binding interface.

Multiple crystal forms of the cell-wall invertase inhibitor from tobacco support high conformational rigidity over a broad pH range.,Hothorn M, Scheffzek K Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):665-70. Epub 2006, May 12. PMID:16699194^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hothorn M, Scheffzek K. Multiple crystal forms of the cell-wall invertase inhibitor from tobacco support high conformational rigidity over a broad pH range. Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):665-70. Epub 2006, May 12. PMID:16699194 doi:10.1107/S0907444906011693

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OCA

[1] Hothorn M, Scheffzek K. Multiple crystal forms of the cell-wall invertase inhibitor from tobacco support high conformational rigidity over a broad pH range. Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):665-70. Epub 2006, May 12. PMID:16699194 doi:10.1107/S0907444906011693

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='2cj4' size='340' side='right'caption='[[2cj4]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2cj4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/American_tobacco American tobacco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CJ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CJ4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2cj4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CJ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CJ4 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.63&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1rj1|1rj1]], [[1rj4|1rj4]], [[2cj5|2cj5]], [[2cj6|2cj6]], [[2cj7|2cj7]], [[2cj8|2cj8]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cj4 OCA], [https://pdbe.org/2cj4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cj4 RCSB], [https://www.ebi.ac.uk/pdbsum/2cj4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cj4 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/O49908_TOBAC O49908_TOBAC]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 31: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: American tobacco]]
 [[Category: Large Structures]]
-[[Category: Hothorn, M]]
+[[Category: Nicotiana tabacum]]
-[[Category: Scheffzek, K]]
+[[Category: Hothorn M]]
-[[Category: Four-helix bundle]]
+[[Category: Scheffzek K]]
-[[Category: Helical hairpin]]
-[[Category: Inhibitor]]
-[[Category: Protein binding]]

2cj4: Difference between revisions

Revision as of 14:19, 13 December 2023

Crystal Structure of a Cell Wall Invertase Inhibitor from Tobacco at pH 4.6

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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