2uul: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2uul]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Phormidium_sp. Phormidium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UUL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UUL FirstGlance]. <br>
<table><tr><td colspan='2'>[[2uul]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Phormidium_sp. Phormidium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UUL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UUL FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLA:BILIVERDINE+IX+ALPHA'>BLA</scene>, <scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1gh0|1gh0]], [[2uum|2uum]], [[2uun|2uun]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLA:BILIVERDINE+IX+ALPHA'>BLA</scene>, <scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uul OCA], [https://pdbe.org/2uul PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uul RCSB], [https://www.ebi.ac.uk/pdbsum/2uul PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uul ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uul OCA], [https://pdbe.org/2uul PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uul RCSB], [https://www.ebi.ac.uk/pdbsum/2uul PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uul ProSAT]</span></td></tr>
</table>
</table>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2uul ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2uul ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
C-phycocyanins from three cyanobacterial cultures of freshwater and marine habitat, Spirulina, Phormidium and Lyngbya spp., were purified to homogeneity and crystallized using the hanging-drop vapour-diffusion method. Blue-coloured crystals in different crystal forms, monoclinic and hexagonal, were obtained for the three species. The crystals took 1-12 weeks to grow to full size using polyethylene glycols of different molecular weights as precipitants. The amino-acid sequences of these proteins show high similarity to other known C-phycocyanins from related organisms; however, the C-phycocyanins reported here showed different biochemical and biophysical properties, i.e. molecular weight, stability etc. The X-ray diffraction data were collected at resolutions of 3.0 A for the monoclinic and 3.2 and 3.6 A for the hexagonal forms. The unit-cell parameters corresponding to the monoclinic space group P2(1) are a = 107.33, b = 115.64, c = 183.26 A, beta = 90.03 degrees for Spirulina sp. C-phycocyanin and are similar for crystals of Phormidium and Lyngbya spp. C-phycocyanins. Crystals belonging to the hexagonal space group P6(3), with unit-cell parameters a = b = 154.97, c = 40.35 A and a = b = 151.96, c = 39.06 A, were also obtained for the C-phycocyanins from Spirulina and Lyngbya spp., respectively. The estimated solvent content is around 50% for the monoclinic crystals of all three species assuming the presence of two hexamers per asymmetric unit. The solvent content is 66.5 and 64.1% for the hexagonal crystals of C-phycocyanin from Spirulina and Lyngbya spp. assuming the presence of one alphabeta monomer per asymmetric unit.
X-ray crystallographic studies on C-phycocyanins from cyanobacteria from different habitats: marine and freshwater.,Satyanarayana L, Suresh CG, Patel A, Mishra S, Ghosh PK Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Sep 1;61(Pt, 9):844-7. Epub 2005 Aug 31. PMID:16511175<ref>PMID:16511175</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2uul" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Phycocyanin|Phycocyanin]]
*[[Phycocyanin|Phycocyanin]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Phormidium sp]]
[[Category: Phormidium sp]]
[[Category: Ghosh, P K]]
[[Category: K Ghosh P]]
[[Category: Mishra, S]]
[[Category: Mishra S]]
[[Category: Patel, A]]
[[Category: Patel A]]
[[Category: Satyanarayana, L]]
[[Category: Satyanarayana L]]
[[Category: Suresh, C G]]
[[Category: Suresh CG]]
[[Category: Bile pigment]]
[[Category: C-phycocyanin]]
[[Category: Chromophore]]
[[Category: Electron transport]]
[[Category: Fresh water]]
[[Category: Lyngbya]]
[[Category: Marine]]
[[Category: Methylation]]
[[Category: Phormidium]]
[[Category: Photosynthesis]]
[[Category: Phycobilisome]]
[[Category: Spirulina]]
[[Category: Transport]]

Revision as of 14:55, 13 December 2023

Crystal structure of C-phycocyanin from Phormidium, Lyngbya spp. (Marine) and Spirulina sp. (Fresh water) shows two different ways of energy transfer between two hexamers.

2uul, resolution 3.10Å

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