2w79

Establishing wild-type levels of catalytic activity on natural and artificial (ba)8-barrel protein scaffolds

spinqualitylabelsall models 2w79, resolution 1.85Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2w79 is a 2 chain structure with sequence from Thermotoga maritima. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.85Å Ligands: CL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function HIS4_THEMA Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The generation of high levels of new catalytic activities on natural and artificial protein scaffolds is a major goal of enzyme engineering. Here, we used random mutagenesis and selection in vivo to establish a sugar isomerisation reaction on both a natural (betaalpha)(8)-barrel enzyme and a catalytically inert chimeric (betaalpha)(8)-barrel scaffold, which was generated by the recombination of 2 (betaalpha)(4)-half barrels. The best evolved variants show turnover numbers and substrate affinities that are similar to those of wild-type enzymes catalyzing the same reaction. The determination of the crystal structure of the most proficient variant allowed us to model the substrate sugar in the novel active site and to elucidate the mechanistic basis of the newly established activity. The results demonstrate that natural and inert artificial protein scaffolds can be converted into highly proficient enzymes in the laboratory, and provide insights into the mechanisms of enzyme evolution. Establishing wild-type levels of catalytic activity on natural and artificial ({beta}{alpha})8-barrel protein scaffolds.,Claren J, Malisi C, Hocker B, Sterner R Proc Natl Acad Sci U S A. 2009 Feb 23. PMID:19237570 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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