5omt

Endonuclease NucB

spinqualitylabelsall models 5omt, resolution 1.35Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5omt is a 1 chain structure with sequence from Bacillus licheniformis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.35Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function F1BV52_BACLI Publication Abstract from PubMed Bacterial biofilms are a complex architecture of cells that grow on moist interfaces, and are held together by a molecular glue of extracellular proteins, sugars and nucleic acids. Biofilms are particularly problematic in human healthcare as they can coat medical implants and are thus a potential source of disease. The enzymatic dispersal of biofilms is increasingly being developed as a new strategy to treat this problem. Here, we have characterized NucB, a biofilm-dispersing nuclease from a marine strain of Bacillus licheniformis, and present its crystal structure together with the biochemistry and a mutational analysis required to confirm its active site. Taken together, these data support the categorization of NucB into a unique subfamily of the betabetaalpha metal-dependent non-specific endonucleases. Understanding the structure and function of NucB will facilitate its future development into an anti-biofilm therapeutic agent. Crystal structure of NucB, a biofilm-degrading endonuclease.,Basle A, Hewitt L, Koh A, Lamb HK, Thompson P, Burgess JG, Hall MJ, Hawkins AR, Murray H, Lewis RJ Nucleic Acids Res. 2017 Nov 20. pii: 4641913. doi: 10.1093/nar/gkx1170. PMID:29165717 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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