2yfh

Structure of a Chimeric Glutamate Dehydrogenase

spinqualitylabelsall models 2yfh, resolution 2.69Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2yfh is a 6 chain structure with sequence from Escherichia coli and Clostridium symbiosum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.695Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function DHE4_ECOLI Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. E7GGS1_CLOSY Publication Abstract from PubMed Glutamate dehydrogenases (EC 1.4.1.2-4) catalyse the oxidative deamination of L-glutamate to alpha-ketoglutarate using NAD(P)(+) as a cofactor. The bacterial enzymes are hexameric, arranged with 32 symmetry, and each polypeptide consists of an N-terminal substrate-binding segment (domain I) followed by a C-terminal cofactor-binding segment (domain II). The catalytic reaction takes place in the cleft formed at the junction of the two domains. Distinct signature sequences in the nucleotide-binding domain have been linked to the binding of NAD(+) versus NADP(+), but they are not unambiguous predictors of cofactor preference. In the absence of substrate, the two domains move apart as rigid bodies, as shown by the apo structure of glutamate dehydrogenase from Clostridium symbiosum. Here, the crystal structure of a chimaeric clostridial/Escherichia coli enzyme has been determined in the apo state. The enzyme is fully functional and reveals possible determinants of interdomain flexibility at a hinge region following the pivot helix. The enzyme retains the preference for NADP(+) cofactor from the parent E. coli domain II, although there are subtle differences in catalytic activity. Crystal structure of a chimaeric bacterial glutamate dehydrogenase.,Oliveira T, Sharkey MA, Engel PC, Khan AR Acta Crystallogr F Struct Biol Commun. 2016 Jun 1;72(Pt 6):462-6. doi:, 10.1107/S2053230X16007305. Epub 2016 May 23. PMID:27303899 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Glutamate dehydrogenase 3D structures

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