4bec: Difference between revisions

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<StructureSection load='4bec' size='340' side='right'caption='[[4bec]], [[Resolution|resolution]] 2.84&Aring;' scene=''>
<StructureSection load='4bec' size='340' side='right'caption='[[4bec]], [[Resolution|resolution]] 2.84&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4bec]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BEC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BEC FirstGlance]. <br>
<table><tr><td colspan='2'>[[4bec]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BEC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BEC FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.84&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4be7|4be7]], [[4beb|4beb]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Type_I_site-specific_deoxyribonuclease Type I site-specific deoxyribonuclease], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.3 3.1.21.3] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bec OCA], [https://pdbe.org/4bec PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bec RCSB], [https://www.ebi.ac.uk/pdbsum/4bec PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bec ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bec OCA], [https://pdbe.org/4bec PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bec RCSB], [https://www.ebi.ac.uk/pdbsum/4bec PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bec ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/T1R1_ECOLX T1R1_ECOLX] The restriction (R) subunit of a type I restriction enzyme that recognizes 5'-GAAN(7)RTCG-3' and cleaves a random distance away. Subunit R is required for both nuclease and ATPase activities, but not for modification (Probable) (PubMed:12654995). After locating an unmethylated recognition site, the enzyme complex serves as a molecular motor that translocates DNA in an ATP-dependent manner until a collision occurs that triggers cleavage (Probable). The enzyme undergoes major structural changes to bring the motor domains into contact with DNA, allowing DNA translocation. This prevents DNA access to the catalytic domains of both the R and M subunits, preventing both restriction and methylation (PubMed:32483229).<ref>PMID:32483229</ref> <ref>PMID:12654995</ref> <ref>PMID:2784505</ref> <ref>PMID:32483229</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bacillus coli migula 1895]]
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Type I site-specific deoxyribonuclease]]
[[Category: Baikova T]]
[[Category: Baikova, T]]
[[Category: Carey J]]
[[Category: Carey, J]]
[[Category: Csefalvay E]]
[[Category: Csefalvay, E]]
[[Category: Csefalvay L]]
[[Category: Csefalvay, L]]
[[Category: Ettrich R]]
[[Category: Ettrich, R]]
[[Category: Guzanova A]]
[[Category: Guzanova, A]]
[[Category: Janscak P]]
[[Category: Janscak, P]]
[[Category: Lapkouski M]]
[[Category: Lapkouski, M]]
[[Category: Panjikar S]]
[[Category: Panjikar, S]]
[[Category: Shevelev I]]
[[Category: Shevelev, I]]
[[Category: Smatanova IK]]
[[Category: Smatanova, I K]]
[[Category: Weiserova M]]
[[Category: Weiserova, M]]
[[Category: Dna modification]]
[[Category: Hydrolase]]
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