4u87

Crystal structure of the Ba-soaked C2 crystal form of pMV158 replication initiator RepB (P3221 space group)

spinqualitylabelsall models 4u87, resolution 3.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4u87 is a 3 chain structure with sequence from Streptococcus agalactiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.8Å Ligands: BA, CL, MN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function REPB_STRAG Is essential for plasmid replication. Nicks the positive strand at the plus origin of replication. Publication Abstract from PubMed DNA replication initiation is a vital and tightly regulated step in all replicons and requires an initiator factor that specifically recognizes the DNA replication origin and starts replication. RepB from the promiscuous streptococcal plasmid pMV158 is a hexameric ring protein evolutionary related to viral initiators. Here we explore the conformational plasticity of the RepB hexamer by i) SAXS, ii) sedimentation experiments, iii) molecular simulations and iv) X-ray crystallography. Combining these techniques, we derive an estimate of the conformational ensemble in solution showing that the C-terminal oligomerisation domains of the protein form a rigid cylindrical scaffold to which the N-terminal DNA-binding/catalytic domains are attached as highly flexible appendages, featuring multiple orientations. In addition, we show that the hinge region connecting both domains plays a pivotal role in the observed plasticity. Sequence comparisons and a literature survey show that this hinge region could exists in other initiators, suggesting that it is a common, crucial structural element for DNA binding and manipulation. Conformational plasticity of RepB, the replication initiator protein of promiscuous streptococcal plasmid pMV158.,Boer DR, Ruiz-Maso JA, Rueda M, Petoukhov MV, Machon C, Svergun DI, Orozco M, Del Solar G, Coll M Sci Rep. 2016 Feb 15;6:20915. doi: 10.1038/srep20915. PMID:26875695 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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