6hlu

Crystal structure of the LRR-Roc-COR domain of the Chlorobium tepidum Roco protein

spinqualitylabelsall models 6hlu, resolution 3.29Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6hlu is a 2 chain structure with sequence from Chlorobaculum tepidum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.29Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q8KC98_CHLTE Publication Abstract from PubMed The LRR-Roc-COR domains are central to the action of nearly all Roco proteins, including the Parkinson's disease-associated protein LRRK2. We previously demonstrated that the Roco protein from Chlorobium tepidum (CtRoco) undergoes a dimer-monomer cycle during the GTPase reaction, with the protein being mainly dimeric in the nucleotide-free and GDP-bound states and monomeric in the GTP-bound state. Here, we report a crystal structure of CtRoco in the nucleotide-free state showing for the first time the arrangement of the LRR-Roc-COR. This structure reveals a compact dimeric arrangement and shows an unanticipated intimate interaction between the Roc GTPase domains in the dimer interface, involving residues from the P-loop, the switch II loop, the G4 region and a loop which we named the "Roc dimerization loop". Hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) is subsequently used to highlight structural alterations induced by individual steps along the GTPase cycle. The structure and HDX-MS data propose a pathway linking nucleotide binding to monomerization and relaying the conformational changes via the Roc switch II to the LRR and COR domains. Together, this work provides important new insights in the regulation of the Roco proteins. Structure and nucleotide-induced conformational dynamics of the Chlorobium tepidum Roco protein.,Deyaert E, Leemans M, Singh RK, Gallardo R, Steyaert J, Kortholt A, Lauer J, Versees W Biochem J. 2018 Dec 11. pii: BCJ20180803. doi: 10.1042/BCJ20180803. PMID:30538153 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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