6s6h: Difference between revisions
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==== | ==Crystal structure of the DNA binding domain of the chromosome-partitioning protein ParB complexed to the centromeric parS site== | ||
<StructureSection load='6s6h' size='340' side='right'caption='[[6s6h]]' scene=''> | <StructureSection load='6s6h' size='340' side='right'caption='[[6s6h]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[6s6h]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Caulobacter_vibrioides Caulobacter vibrioides] and [https://en.wikipedia.org/wiki/Caulobacter_vibrioides_NA1000 Caulobacter vibrioides NA1000]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S6H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6S6H FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6s6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s6h OCA], [https://pdbe.org/6s6h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6s6h RCSB], [https://www.ebi.ac.uk/pdbsum/6s6h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6s6h ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6s6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s6h OCA], [https://pdbe.org/6s6h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6s6h RCSB], [https://www.ebi.ac.uk/pdbsum/6s6h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6s6h ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/PARB_CAUVN PARB_CAUVN] Involved in chromosome partition. Localize to both poles of the predivisional cell following completion of DNA replication. Binds to the DNA origin of replication.<ref>PMID:9054507</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Specific interactions between proteins and DNA are essential to many biological processes. Yet, it remains unclear how the diversification in DNA-binding specificity was brought about, and the mutational paths that led to changes in specificity are unknown. Using a pair of evolutionarily related DNA-binding proteins, each with a different DNA preference (ParB [Partitioning Protein B] and Noc [Nucleoid Occlusion Factor], which both play roles in bacterial chromosome maintenance), we show that specificity is encoded by a set of four residues at the protein-DNA interface. Combining X-ray crystallography and deep mutational scanning of the interface, we suggest that permissive mutations must be introduced before specificity-switching mutations to reprogram specificity and that mutational paths to new specificity do not necessarily involve dual-specificity intermediates. Overall, our results provide insight into the possible evolutionary history of ParB and Noc and, in a broader context, might be useful for understanding the evolution of other classes of DNA-binding proteins. | |||
Diversification of DNA-Binding Specificity by Permissive and Specificity-Switching Mutations in the ParB/Noc Protein Family.,Jalal ASB, Tran NT, Stevenson CE, Chan EW, Lo R, Tan X, Noy A, Lawson DM, Le TBK Cell Rep. 2020 Jul 21;32(3):107928. doi: 10.1016/j.celrep.2020.107928. PMID:32698006<ref>PMID:32698006</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6s6h" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Caulobacter vibrioides]] | |||
[[Category: Caulobacter vibrioides NA1000]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Jalal ASB]] | ||
[[Category: Lawson DM]] | |||
[[Category: Le TBK]] | |||
[[Category: Stevenson CEM]] | |||
[[Category: Tan EX]] | |||
[[Category: Tran NT]] | |||
