6ttj: Difference between revisions

Latest revision as of 13:09, 24 January 2024

Neutral invertase 2 from Arabidopsis thaliana

Structural highlights

6ttj is a 12 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.392Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CINV1_ARATH Cytosolic invertase that specifically cleaves sucrose into glucose and fructose and is involved in the regulation of multiple tissue development including primary root elongation, root hair growth, leaf and silique development, and floral transition. Is involved in osmotic stress-induced inhibition on lateral root growth by controlling the concentration of hexose in cells. May regulate sugar-mediated root development by controlling sucrose catabolism in root cells.^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

The metabolism of sucrose is of crucial importance for life on Earth. In plants, enzymes called invertases split sucrose into glucose and fructose, contributing to the regulation of metabolic fluxes. Invertases differ in their localization and pH optimum. Acidic invertases present in plant cell walls and vacuoles belong to glycoside hydrolase family 32 (GH32) and have an all-beta structure. In contrast, neutral invertases are located in the cytosol and organelles such as chloroplasts and mitochondria. These poorly understood enzymes are classified into a separate GH100 family. Recent crystal structures of the closely related neutral invertases InvA and InvB from the cyanobacterium Anabaena revealed a predominantly alpha-helical fold with unique features compared with other sucrose-metabolizing enzymes. Here, a neutral invertase (AtNIN2) from the model plant Arabidopsis thaliana was heterologously expressed, purified and crystallized. As a result, the first neutral invertase structure from a higher plant has been obtained at 3.4 A resolution. The hexameric AtNIN2 structure is highly similar to that of InvA, pointing to high evolutionary conservation of neutral invertases.

Crystal structure of Arabidopsis thaliana neutral invertase 2.,Tarkowski LP, Tsirkone VG, Osipov EM, Beelen S, Lammens W, Vergauwen R, Van den Ende W, Strelkov SV Acta Crystallogr F Struct Biol Commun. 2020 Mar 1;76(Pt 3):152-157. doi:, 10.1107/S2053230X2000179X. Epub 2020 Mar 3. PMID:32134001^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lou Y, Gou JY, Xue HW. PIP5K9, an Arabidopsis phosphatidylinositol monophosphate kinase, interacts with a cytosolic invertase to negatively regulate sugar-mediated root growth. Plant Cell. 2007 Jan;19(1):163-81. Epub 2007 Jan 12. PMID:17220200 doi:https://dx.doi.org/tpc.106.045658
↑ Qi X, Wu Z, Li J, Mo X, Wu S, Chu J, Wu P. AtCYT-INV1, a neutral invertase, is involved in osmotic stress-induced inhibition on lateral root growth in Arabidopsis. Plant Mol Biol. 2007 Jul;64(5):575-87. doi: 10.1007/s11103-007-9177-4. Epub 2007 , May 17. PMID:17508130 doi:https://dx.doi.org/10.1007/s11103-007-9177-4
↑ Barratt DH, Derbyshire P, Findlay K, Pike M, Wellner N, Lunn J, Feil R, Simpson C, Maule AJ, Smith AM. Normal growth of Arabidopsis requires cytosolic invertase but not sucrose synthase. Proc Natl Acad Sci U S A. 2009 Aug 4;106(31):13124-9. doi:, 10.1073/pnas.0900689106. Epub 2009 May 21. PMID:19470642 doi:https://dx.doi.org/10.1073/pnas.0900689106
↑ Xiang L, Le Roy K, Bolouri-Moghaddam MR, Vanhaecke M, Lammens W, Rolland F, Van den Ende W. Exploring the neutral invertase-oxidative stress defence connection in Arabidopsis thaliana. J Exp Bot. 2011 Jul;62(11):3849-62. doi: 10.1093/jxb/err069. Epub 2011 Mar 25. PMID:21441406 doi:https://dx.doi.org/10.1093/jxb/err069
↑ Tarkowski LP, Tsirkone VG, Osipov EM, Beelen S, Lammens W, Vergauwen R, Van den Ende W, Strelkov SV. Crystal structure of Arabidopsis thaliana neutral invertase 2. Acta Crystallogr F Struct Biol Commun. 2020 Mar 1;76(Pt 3):152-157. doi:, 10.1107/S2053230X2000179X. Epub 2020 Mar 3. PMID:32134001 doi:https://dx.doi.org/10.1107/S2053230X2000179X

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Lou Y, Gou JY, Xue HW. PIP5K9, an Arabidopsis phosphatidylinositol monophosphate kinase, interacts with a cytosolic invertase to negatively regulate sugar-mediated root growth. Plant Cell. 2007 Jan;19(1):163-81. Epub 2007 Jan 12. PMID:17220200 doi:https://dx.doi.org/tpc.106.045658

[2] Qi X, Wu Z, Li J, Mo X, Wu S, Chu J, Wu P. AtCYT-INV1, a neutral invertase, is involved in osmotic stress-induced inhibition on lateral root growth in Arabidopsis. Plant Mol Biol. 2007 Jul;64(5):575-87. doi: 10.1007/s11103-007-9177-4. Epub 2007 , May 17. PMID:17508130 doi:https://dx.doi.org/10.1007/s11103-007-9177-4

[3] Barratt DH, Derbyshire P, Findlay K, Pike M, Wellner N, Lunn J, Feil R, Simpson C, Maule AJ, Smith AM. Normal growth of Arabidopsis requires cytosolic invertase but not sucrose synthase. Proc Natl Acad Sci U S A. 2009 Aug 4;106(31):13124-9. doi:, 10.1073/pnas.0900689106. Epub 2009 May 21. PMID:19470642 doi:https://dx.doi.org/10.1073/pnas.0900689106

[4] Xiang L, Le Roy K, Bolouri-Moghaddam MR, Vanhaecke M, Lammens W, Rolland F, Van den Ende W. Exploring the neutral invertase-oxidative stress defence connection in Arabidopsis thaliana. J Exp Bot. 2011 Jul;62(11):3849-62. doi: 10.1093/jxb/err069. Epub 2011 Mar 25. PMID:21441406 doi:https://dx.doi.org/10.1093/jxb/err069

[5] Tarkowski LP, Tsirkone VG, Osipov EM, Beelen S, Lammens W, Vergauwen R, Van den Ende W, Strelkov SV. Crystal structure of Arabidopsis thaliana neutral invertase 2. Acta Crystallogr F Struct Biol Commun. 2020 Mar 1;76(Pt 3):152-157. doi:, 10.1107/S2053230X2000179X. Epub 2020 Mar 3. PMID:32134001 doi:https://dx.doi.org/10.1107/S2053230X2000179X

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[5]

@@ Line 3: / Line 3: @@
 <StructureSection load='6ttj' size='340' side='right'caption='[[6ttj]], [[Resolution|resolution]] 3.39&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6ttj]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TTJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6TTJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6ttj]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TTJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TTJ FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6sgq|6sgq]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.392&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CINV1, INVG, At1g35580, F15O4.33 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ttj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ttj OCA], [https://pdbe.org/6ttj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ttj RCSB], [https://www.ebi.ac.uk/pdbsum/6ttj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ttj ProSAT]</span></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-fructofuranosidase Beta-fructofuranosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.26 3.2.1.26] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ttj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ttj OCA], [http://pdbe.org/6ttj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ttj RCSB], [http://www.ebi.ac.uk/pdbsum/6ttj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ttj ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CINV1_ARATH CINV1_ARATH]] Cytosolic invertase that specifically cleaves sucrose into glucose and fructose and is involved in the regulation of multiple tissue development including primary root elongation, root hair growth, leaf and silique development, and floral transition. Is involved in osmotic stress-induced inhibition on lateral root growth by controlling the concentration of hexose in cells. May regulate sugar-mediated root development by controlling sucrose catabolism in root cells.<ref>PMID:17220200</ref> <ref>PMID:17508130</ref> <ref>PMID:19470642</ref> <ref>PMID:21441406</ref>
+[https://www.uniprot.org/uniprot/CINV1_ARATH CINV1_ARATH] Cytosolic invertase that specifically cleaves sucrose into glucose and fructose and is involved in the regulation of multiple tissue development including primary root elongation, root hair growth, leaf and silique development, and floral transition. Is involved in osmotic stress-induced inhibition on lateral root growth by controlling the concentration of hexose in cells. May regulate sugar-mediated root development by controlling sucrose catabolism in root cells.<ref>PMID:17220200</ref> <ref>PMID:17508130</ref> <ref>PMID:19470642</ref> <ref>PMID:21441406</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 18: @@
 </div>
 <div class="pdbe-citations 6ttj" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Invertase|Invertase]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Arath]]
+[[Category: Arabidopsis thaliana]]
-[[Category: Beta-fructofuranosidase]]
 [[Category: Large Structures]]
-[[Category: Beelen, S]]
+[[Category: Beelen S]]
-[[Category: Osipov, E M]]
+[[Category: Osipov EM]]
-[[Category: Strelkov, S V]]
+[[Category: Strelkov SV]]
-[[Category: Tsirkone, V G]]
+[[Category: Tsirkone VG]]
-[[Category: Carbohydrate]]
-[[Category: Hydrolase]]
-[[Category: Invertase]]

6ttj: Difference between revisions

Latest revision as of 13:09, 24 January 2024

Neutral invertase 2 from Arabidopsis thaliana

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

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Proteopedia Page Contributors and Editors (what is this?)

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