6yji: Difference between revisions

Latest revision as of 13:28, 24 January 2024

Structure of FgCelDH7C

Structural highlights

6yji is a 2 chain structure with sequence from Fusarium graminearum PH-1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.64Å
Ligands:	1PE, CL, FAD, NAG, PEG, PG4, SCN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

I1RWY4_GIBZE

Publication Abstract from PubMed

Oxidative plant cell-wall processing enzymes are of great importance in biology and biotechnology. Yet, our insight into the functional interplay amongst such oxidative enzymes remains limited. Here, a phylogenetic analysis of the auxiliary activity 7 family (AA7), currently harbouring oligosaccharide flavo-oxidases, reveals a striking abundance of AA7-genes in phytopathogenic fungi and Oomycetes. Expression of five fungal enzymes, including three from unexplored clades, expands the AA7-substrate range and unveils a cellooligosaccharide dehydrogenase activity, previously unknown within AA7. Sequence and structural analyses identify unique signatures distinguishing the strict dehydrogenase clade from canonical AA7 oxidases. The discovered dehydrogenase directly is able to transfer electrons to an AA9 lytic polysaccharide monooxygenase (LPMO) and fuel cellulose degradation by LPMOs without exogenous reductants. The expansion of redox-profiles and substrate range highlights the functional diversity within AA7 and sets the stage for harnessing AA7 dehydrogenases to fine-tune LPMO activity in biotechnological conversion of plant feedstocks.

Discovery of fungal oligosaccharide-oxidising flavo-enzymes with previously unknown substrates, redox-activity profiles and interplay with LPMOs.,Haddad Momeni M, Fredslund F, Bissaro B, Raji O, Vuong TV, Meier S, Nielsen TS, Lombard V, Guigliarelli B, Biaso F, Haon M, Grisel S, Henrissat B, Welner DH, Master ER, Berrin JG, Abou Hachem M Nat Commun. 2021 Apr 9;12(1):2132. doi: 10.1038/s41467-021-22372-0. PMID:33837197^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Haddad Momeni M, Fredslund F, Bissaro B, Raji O, Vuong TV, Meier S, Nielsen TS, Lombard V, Guigliarelli B, Biaso F, Haon M, Grisel S, Henrissat B, Welner DH, Master ER, Berrin JG, Abou Hachem M. Discovery of fungal oligosaccharide-oxidising flavo-enzymes with previously unknown substrates, redox-activity profiles and interplay with LPMOs. Nat Commun. 2021 Apr 9;12(1):2132. doi: 10.1038/s41467-021-22372-0. PMID:33837197 doi:https://dx.doi.org/10.1038/s41467-021-22372-0

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Haddad Momeni M, Fredslund F, Bissaro B, Raji O, Vuong TV, Meier S, Nielsen TS, Lombard V, Guigliarelli B, Biaso F, Haon M, Grisel S, Henrissat B, Welner DH, Master ER, Berrin JG, Abou Hachem M. Discovery of fungal oligosaccharide-oxidising flavo-enzymes with previously unknown substrates, redox-activity profiles and interplay with LPMOs. Nat Commun. 2021 Apr 9;12(1):2132. doi: 10.1038/s41467-021-22372-0. PMID:33837197 doi:https://dx.doi.org/10.1038/s41467-021-22372-0

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='6yji' size='340' side='right'caption='[[6yji]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6yji]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_graminearum Fusarium graminearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YJI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YJI FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6yji]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_graminearum_PH-1 Fusarium graminearum PH-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YJI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YJI FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.64&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FG08824.1, FGRAMPH1_01T10923 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=229533 Fusarium graminearum])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yji OCA], [https://pdbe.org/6yji PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yji RCSB], [https://www.ebi.ac.uk/pdbsum/6yji PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yji ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/I1RWY4_GIBZE I1RWY4_GIBZE]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Fusarium graminearum]]
+[[Category: Fusarium graminearum PH-1]]
 [[Category: Large Structures]]
-[[Category: Berrin, J G]]
+[[Category: Abou Hachem M]]
-[[Category: Fredslund, F]]
+[[Category: Berrin JG]]
-[[Category: Hachem, M Abou]]
+[[Category: Fredslund F]]
-[[Category: Momeni, M Haddad]]
+[[Category: Haddad Momeni M]]
-[[Category: Welner, D H]]
+[[Category: Welner DH]]
-[[Category: Fad]]
-[[Category: Linking]]
-[[Category: Oxidoreductase]]

6yji: Difference between revisions

Latest revision as of 13:28, 24 January 2024

Structure of FgCelDH7C

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools