6z6b: Difference between revisions
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==== | ==Structure of full-length La Crosse virus L protein (polymerase)== | ||
<StructureSection load='6z6b' size='340' side='right'caption='[[6z6b]]' scene=''> | <StructureSection load='6z6b' size='340' side='right'caption='[[6z6b]], [[Resolution|resolution]] 3.96Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[6z6b]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/La_Crosse_orthobunyavirus La Crosse orthobunyavirus] and [https://en.wikipedia.org/wiki/La_Crosse_virus La Crosse virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z6B FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.961Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z6b OCA], [https://pdbe.org/6z6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z6b RCSB], [https://www.ebi.ac.uk/pdbsum/6z6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z6b ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/L_BUNLC L_BUNLC] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bunyavirales is an order of segmented negative-strand RNA viruses comprising several life-threatening pathogens against which no effective treatment is currently available. Replication and transcription of the RNA genome constitute essential processes performed by the virally encoded multi-domain RNA-dependent RNA polymerase. Here, we describe the complete high-resolution cryo-EM structure of La Crosse virus polymerase. It reveals the presence of key protruding C-terminal domains, notably the cap-binding domain, which undergoes large movements related to its role in transcription initiation, and a zinc-binding domain that displays a fold not previously observed. We capture the polymerase structure at pre-initiation and elongation states, uncovering the coordinated movement of the priming loop, mid-thumb ring linker and lid domain required for the establishment of a ten-base-pair template-product RNA duplex before strand separation into respective exit tunnels. These structural details and the observed dynamics of key functional elements will be instrumental for structure-based development of polymerase inhibitors. | |||
Pre-initiation and elongation structures of full-length La Crosse virus polymerase reveal functionally important conformational changes.,Arragain B, Effantin G, Gerlach P, Reguera J, Schoehn G, Cusack S, Malet H Nat Commun. 2020 Jul 17;11(1):3590. doi: 10.1038/s41467-020-17349-4. PMID:32681014<ref>PMID:32681014</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6z6b" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: La Crosse orthobunyavirus]] | |||
[[Category: La Crosse virus]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Cusack S]] | ||
[[Category: Gerlach P]] | |||
[[Category: Reguera J]] | |||
Latest revision as of 13:42, 24 January 2024
Structure of full-length La Crosse virus L protein (polymerase)
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