3j0r

Model of a type III secretion system needle based on a 7 Angstrom resolution cryoEM map

Loading... Status: waiting for Mol* initialization Structural highlights 3j0r is a 1 chain structure with sequence from Shigella flexneri. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 7.7Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function MXIH_SHIFL Necessary for the secretion of IPA invasins. Publication Abstract from PubMed Type III secretion systems of Gram-negative bacteria form injection devices that deliver effector proteins into eukaryotic cells during infection. They span both bacterial membranes and the extracellular space to connect with the host cell plasma membrane. Their extracellular portion is a needle-like, hollow tube that serves as a secretion conduit for effector proteins. The needle of Shigella flexneri is approximately 50-nm long and 7-nm thick and is made by the helical assembly of one protein, MxiH. We provide a 7-A resolution 3D image reconstruction of the Shigella needle by electron cryomicroscopy, which resolves alpha-helices and a beta-hairpin that has never been observed in the crystal and solution structures of needle proteins, including MxiH. An atomic model of the needle based on the 3D-density map, in comparison with that of the bacterial-flagellar filament, provides insights into how such a thin tubular structure is stably assembled by intricate intermolecular interactions. The map also illuminates how the needle-length control protein functions as a ruler within the central channel during export of MxiH for assembly at the distal end of the needle, and how the secretion-activation signal may be transduced through a conformational change of the needle upon host-cell contact. Structure of a type III secretion needle at 7-A resolution provides insights into its assembly and signaling mechanisms.,Fujii T, Cheung M, Blanco A, Kato T, Blocker AJ, Namba K Proc Natl Acad Sci U S A. 2012 Mar 2. PMID:22388746 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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