8wbr: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
Line 1: Line 1:
'''Unreleased structure'''


The entry 8wbr is ON HOLD  until 2025-09-10
==Crystal structure of cis-Epoxysuccinate Hydrolases KlCESH[L]==
<StructureSection load='8wbr' size='340' side='right'caption='[[8wbr]], [[Resolution|resolution]] 2.02&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8wbr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_sp._BK-58 Klebsiella sp. BK-58]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WBR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WBR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.02&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8wbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8wbr OCA], [https://pdbe.org/8wbr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8wbr RCSB], [https://www.ebi.ac.uk/pdbsum/8wbr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8wbr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/W8PFD2_KLEOX W8PFD2_KLEOX] Catalyzes the hydrolytic dehalogenation of small (S)-2-haloalkanoic acids to yield the corresponding (R)-2-hydroxyalkanoic acids.[RuleBase:RU368077]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Microbial epoxide hydrolases, cis-Epoxysuccinate hydrolases (CESHs) have been utilized for commercial production of enantiomerically pure L(+)- and D(-)-tartaric acids for decades. However, the stereo-catalytic mechanism of CESH producing L(+)-tartaric acid (CESH[L]) remains unclear. Herein, the crystal structures of two CESH[L]s in ligand-free, product-complexed, and catalytic intermediate forms were determined. These structures revealed the unique specific binding mode for the mirror-symmetric substrate, an active catalytic triad consisting of Asp-His-Glu, and an arginine providing a proton to the oxirane oxygen to facilitate the epoxide ring-opening reaction, which has been pursued for decades. These results provide the structural basis for the rational engineering of these industrial biocatalysts.


Authors: Dong, S., Xuan, J.S., Feng, Y.G., Cui, Q.
Deciphering the stereo-specific catalytic mechanisms of cis-epoxysuccinate hydrolases producing L(+)-tartaric acid.,Dong S, Xuan J, Feng Y, Cui Q J Biol Chem. 2024 Jan 8:105635. doi: 10.1016/j.jbc.2024.105635. PMID:38199576<ref>PMID:38199576</ref>


Description: Crystal structure of cis-Epoxysuccinate Hydrolases KlCESH[L]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Dong, S]]
<div class="pdbe-citations 8wbr" style="background-color:#fffaf0;"></div>
[[Category: Xuan, J.S]]
== References ==
[[Category: Cui, Q]]
<references/>
[[Category: Feng, Y.G]]
__TOC__
</StructureSection>
[[Category: Klebsiella sp. BK-58]]
[[Category: Large Structures]]
[[Category: Cui Q]]
[[Category: Dong S]]
[[Category: Feng YG]]
[[Category: Xuan JS]]
Retrieved from "https://proteopedia.org/8wbr"