6ztx

Crystal Structure of catalase HPII from Escherichia coli (serendipitously crystallized)

spinqualitylabelsall models 6ztx, resolution 1.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6ztx is a 4 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.3Å Ligands: EDO, GOL, HDD Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CATE_ECOLI Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide. Publication Abstract from PubMed Protein crystallographers are well aware of the trap of crystallizing E. coli proteins instead of the macromolecule of interest if heterologous recombinant protein expression in E. coli was part of the experimental pipeline. Among the well-known culprits are YodA metal-binding lipocalin (25 kDa) and YadF carbonic anhydrase (a tetramer of 25 kDa subunits). We report a novel crystal form of another such culprit, E. coli HPII catalase, which is a tetrameric protein of ~340 kDa molecular weight. HPII is likely to contaminate recombinant protein samples, co-purify, and then co-crystallize with the target proteins, especially if their masses in size exclusion chromatography are ~300-400 kDa. What makes this case more interesting but also parlous, is the fact that HPII can crystallize from very low concentrations, even well below 1 mg/mL. Serendipitous crystallization of E. coli HPII catalase, a sequel to "the tale usually not told".,Grzechowiak M, Sekula B, Jaskolski M, Ruszkowski M Acta Biochim Pol. 2021 Jan 24. pii: 5501. doi: 10.18388/abp.2020_5501. PMID:33485289 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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