7o0n

Crystal structure of a ParB E93A mutant from Myxococcus xanthus bound to CDP and monothiophosphate

spinqualitylabelsall models 7o0n, resolution 1.89Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7o0n is a 2 chain structure with sequence from Myxococcus xanthus DK 1622. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.89Å Ligands: CDP, GOL, MG, PEG, TS6 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q1CVJ4_MYXXD Publication Abstract from PubMed ParB-like CTPases mediate the segregation of bacterial chromosomes and low-copy number plasmids. They act as DNA-sliding clamps that are loaded at parS motifs in the centromere of target DNA molecules and spread laterally to form large nucleoprotein complexes serving as docking points for the DNA segregation machinery. Here, we solve crystal structures of ParB in the pre- and post-hydrolysis state and illuminate the catalytic mechanism of nucleotide hydrolysis. Moreover, we identify conformational changes that underlie the CTP- and parS-dependent closure of ParB clamps. The study of CTPase-deficient ParB variants reveals that CTP hydrolysis serves to limit the sliding time of ParB clamps and thus drives the establishment of a well-defined ParB diffusion gradient across the centromere whose dynamics are critical for DNA segregation. These findings clarify the role of the ParB CTPase cycle in partition complex assembly and function and thus advance our understanding of this prototypic CTP-dependent molecular switch. The CTPase activity of ParB determines the size and dynamics of prokaryotic DNA partition complexes.,Osorio-Valeriano M, Altegoer F, Das CK, Steinchen W, Panis G, Connolley L, Giacomelli G, Feddersen H, Corrales-Guerrero L, Giammarinaro PI, Hanssmann J, Bramkamp M, Viollier PH, Murray S, Schafer LV, Bange G, Thanbichler M Mol Cell. 2021 Sep 18. pii: S1097-2765(21)00738-3. doi:, 10.1016/j.molcel.2021.09.004. PMID:34562373 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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