1dj3: Difference between revisions

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<StructureSection load='1dj3' size='340' side='right'caption='[[1dj3]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
<StructureSection load='1dj3' size='340' side='right'caption='[[1dj3]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1dj3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DJ3 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1dj3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DJ3 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1dj2|1dj2]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dj3 OCA], [https://pdbe.org/1dj3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dj3 RCSB], [https://www.ebi.ac.uk/pdbsum/1dj3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dj3 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dj3 OCA], [https://pdbe.org/1dj3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dj3 RCSB], [https://www.ebi.ac.uk/pdbsum/1dj3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dj3 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/PURA_WHEAT PURA_WHEAT]] Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP (By similarity).  
[https://www.uniprot.org/uniprot/PURA_WHEAT PURA_WHEAT] Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP (By similarity).
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dj3 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dj3 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Catalyzing the first step in the de novo synthesis of adenylmonophosphate, adenylosuccinate synthetase (AdSS) is a known target for herbicides and antibiotics. We have purified and crystallized recombinant AdSS from Arabidopsis thaliana and Tritium aestivum, expressed in Escherichia coli. The structures of A. thaliana and T. aestivum AdSS in complex with GDP were solved at 2.9 A and 3.0 A resolution, respectively. Comparison with the known structures from E. coli reveals that the overall fold is very similar to that of the E. coli protein. The longer N terminus in the plant sequences is at the same place as the longer C terminus of the E. coli sequence in the 3D structure. The GDP-binding sites have one additional hydrogen-bonding partner, which is a plausible explanation for the lower K(m) value. Due to its special position, this partner may also enable GTP to initiate a conformational change, which was, in E. coli AdSS, exclusively activated by ligands at the IMP-binding site. The dimer interfaces show up to six hydrogen bonds and six salt-bridges more than in the E. coli structure, although the contact areas have approximately the same size.
Structures of adenylosuccinate synthetase from Triticum aestivum and Arabidopsis thaliana.,Prade L, Cowan-Jacob SW, Chemla P, Potter S, Ward E, Fonne-Pfister R J Mol Biol. 2000 Feb 18;296(2):569-77. PMID:10669609<ref>PMID:10669609</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1dj3" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Adenylosuccinate synthetase 3D structures|Adenylosuccinate synthetase 3D structures]]
*[[Adenylosuccinate synthetase 3D structures|Adenylosuccinate synthetase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Adenylosuccinate synthase]]
[[Category: Canadian hard winter wheat]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Chemla, P]]
[[Category: Triticum aestivum]]
[[Category: Cowan-Jacob, S W]]
[[Category: Chemla P]]
[[Category: Fonne-Pfister, R]]
[[Category: Cowan-Jacob SW]]
[[Category: Potter, S]]
[[Category: Fonne-Pfister R]]
[[Category: Prade, L]]
[[Category: Potter S]]
[[Category: Ward, E]]
[[Category: Prade L]]
[[Category: Gdp]]
[[Category: Ward E]]
[[Category: Ligase]]
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