8ay3: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[8ay3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [https://en.wikipedia.org/wiki/Citrus_sinensis Citrus sinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AY3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AY3 FirstGlance]. <br>
<table><tr><td colspan='2'>[[8ay3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [https://en.wikipedia.org/wiki/Citrus_sinensis Citrus sinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AY3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AY3 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=OE3:~{N}-[(1-ethyl-4-methyl-2-oxidanylidene-quinolin-6-yl)methyl]benzenesulfonamide'>OE3</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=OE3:~{N}-[(1-ethyl-4-methyl-2-oxidanylidene-quinolin-6-yl)methyl]benzenesulfonamide'>OE3</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ay3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ay3 OCA], [https://pdbe.org/8ay3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ay3 RCSB], [https://www.ebi.ac.uk/pdbsum/8ay3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ay3 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ay3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ay3 OCA], [https://pdbe.org/8ay3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ay3 RCSB], [https://www.ebi.ac.uk/pdbsum/8ay3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ay3 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[https://www.uniprot.org/uniprot/A0A067E666_CITSI A0A067E666_CITSI]  
[https://www.uniprot.org/uniprot/A0A067E666_CITSI A0A067E666_CITSI]  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Strategies to activate abscisic acid (ABA) receptors and boost ABA signaling by small molecules that act as ABA receptor agonists are promising biotechnological tools to enhance plant drought tolerance. Protein structures of crop ABA receptors might require modifications to improve recognition of chemical ligands, which in turn can be optimized by structural information. Through structure-based targeted design, we have combined chemical and genetic approaches to generate an ABA receptor agonist molecule (iSB09) and engineer a CsPYL1 ABA receptor, named CsPYL1(5m), which efficiently binds iSB09. This optimized receptor-agonist pair leads to activation of ABA signaling and marked drought tolerance. No constitutive activation of ABA signaling and hence growth penalty was observed in transformed Arabidopsis thaliana plants. Therefore, conditional and efficient activation of ABA signaling was achieved through a chemical-genetic orthogonal approach based on iterative cycles of ligand and receptor optimization driven by the structure of ternary receptor-ligand-phosphatase complexes.


Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought.,Lozano-Juste J, Infantes L, Garcia-Maquilon I, Ruiz-Partida R, Merilo E, Benavente JL, Velazquez-Campoy A, Coego A, Bono M, Forment J, Pampin B, Destito P, Monteiro A, Rodriguez R, Cruces J, Rodriguez PL, Albert A Sci Adv. 2023 Mar 10;9(10):eade9948. doi: 10.1126/sciadv.ade9948. Epub 2023 Mar , 10. PMID:36897942<ref>PMID:36897942</ref>
==See Also==
 
*[[Protein phosphatase 3D structures|Protein phosphatase 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8ay3" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>

Latest revision as of 08:14, 7 February 2024

X-RAY CRYSTAL STRUCTURE OF THE CsPYL1-iSB9-HAB1 TERNARY COMPLEX

8ay3, resolution 1.90Å

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