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==The independent structure of the antitryptic reactive site loop of Bowman-Birk inhibitor and sunflower trypsin inhibitor-1==
==The independent structure of the antitryptic reactive site loop of Bowman-Birk inhibitor and sunflower trypsin inhibitor-1==
<StructureSection load='1gm2' size='340' side='right'caption='[[1gm2]], [[NMR_Ensembles_of_Models | 30 NMR models]]' scene=''>
<StructureSection load='1gm2' size='340' side='right'caption='[[1gm2]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1gm2]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GM2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GM2 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1gm2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Macrotyloma_axillare Macrotyloma axillare]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GM2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GM2 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gm2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gm2 OCA], [https://pdbe.org/1gm2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gm2 RCSB], [https://www.ebi.ac.uk/pdbsum/1gm2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gm2 ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gm2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gm2 OCA], [https://pdbe.org/1gm2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gm2 RCSB], [https://www.ebi.ac.uk/pdbsum/1gm2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gm2 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/IBB4_MACAX IBB4_MACAX]
Bowman-Birk inhibitor (BBI) proteins contain an inhibitory motif comprising a disulfide-bonded sequence that interacts with serine proteinases. Recently, a small 14-residue peptide from sunflowers (SFTI-1), which has potent anti-trypsin activity, has been found to have the same motif. However, this peptide also has an unusual head-to-tail cyclisation. To address the role of the core inhibitory sequence itself, we have solved the (1)H-NMR solution structure of an antitryptic 11-residue cyclic peptide that corresponds to the core reactive site loops of both SFTI-1 and Bowman-Birk inhibitor proteins. A comparison is made between the secondary chemical shifts found in this family and the canonical regions of several other inhibitors, giving some insight into relative flexibility and hydrogen bonding patterns in these inhibitors. The solution structure of the core peptide in isolation is found to retain essentially the same three-dimensional arrangement of both backbone and side chains as observed in larger antitryptic BBI and SFTI-1 fragments as well as in the complete proteins. The retention of the canonical conformation in the core peptide explains the peptids inhibitory potency. It therefore represents a minimization of both the BBI and SFTI-1 sequences. We conclude that the core peptide is a conformationally defined, canonical scaffold, which can serve as a minimal platform for the engineering of biological activity.
 
The (1)H-NMR solution structure of the antitryptic core peptide of Bowman-Birk inhibitor proteins: a minimal canonical loop.,Brauer AB, Kelly G, Matthews SJ, Leatherbarrow RJ J Biomol Struct Dyn. 2002 Aug;20(1):59-70. PMID:12144352<ref>PMID:12144352</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1gm2" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Brauer, A B.E]]
[[Category: Macrotyloma axillare]]
[[Category: Kelly, G]]
[[Category: Brauer ABE]]
[[Category: Leatherbarrow, R J]]
[[Category: Kelly G]]
[[Category: Matthews, S J]]
[[Category: Leatherbarrow RJ]]
[[Category: Bowman-birk inhibitor protein mimetic]]
[[Category: Matthews SJ]]
[[Category: Hydrolase inhibitor]]
[[Category: Trypsin inhibitor]]
[[Category: Type vib beta-turn peptide]]

Revision as of 08:37, 7 February 2024

The independent structure of the antitryptic reactive site loop of Bowman-Birk inhibitor and sunflower trypsin inhibitor-1

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