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==GA-79-MBP cs-rosetta structures==
==GA-79-MBP cs-rosetta structures==
<StructureSection load='2mh8' size='340' side='right'caption='[[2mh8]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
<StructureSection load='2mh8' size='340' side='right'caption='[[2mh8]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2mh8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"group_ii"_minett_1934 "group ii" minett 1934]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MH8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MH8 FirstGlance]. <br>
<table><tr><td colspan='2'>[[2mh8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_dysgalactiae Streptococcus dysgalactiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MH8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MH8 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mh8 OCA], [https://pdbe.org/2mh8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mh8 RCSB], [https://www.ebi.ac.uk/pdbsum/2mh8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mh8 ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mh8 OCA], [https://pdbe.org/2mh8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mh8 RCSB], [https://www.ebi.ac.uk/pdbsum/2mh8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mh8 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/A0A0J9X1W7_STRDY A0A0J9X1W7_STRDY]
Metamorphic proteins, including proteins with high levels of sequence identity but different folds, are exceptions to the long-standing rule-of-thumb that proteins with as little as 30% sequence identity adopt the same fold. Which topologies can be bridged by these highly identical sequences remains an open question. Here we bridge two 3-alpha-helix bundle proteins with two radically different folds. Using a straightforward approach, we engineered the sequences of one subdomain within maltose binding protein (MBP, alpha/beta/alpha-sandwich) and another within outer surface protein A (OspA, beta-sheet) to have high sequence identity (80 and 77%, respectively) with engineered variants of protein G (GA, 3-alpha-helix bundle). Circular dichroism and nuclear magnetic resonance spectra of all engineered variants demonstrate that they maintain their native conformations despite substantial sequence modification. Furthermore, the MBP variant (80% identical to GA) remained active. Thermodynamic analysis of numerous GA and MBP variants suggests that the key to our approach involved stabilizing the modified MBP and OspA subdomains via external interactions with neighboring substructures, indicating that subdomain interactions can stabilize alternative folds over a broad range of sequence variation. These findings suggest that it is possible to bridge one fold with many other topologies, which has implications for protein folding, evolution, and misfolding diseases.
 
Subdomain interactions foster the design of two protein pairs with approximately 80% sequence identity but different folds.,Porter LL, He Y, Chen Y, Orban J, Bryan PN Biophys J. 2015 Jan 6;108(1):154-62. doi: 10.1016/j.bpj.2014.10.073. PMID:25564862<ref>PMID:25564862</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2mh8" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Group ii minett 1934]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Bryan, P]]
[[Category: Streptococcus dysgalactiae]]
[[Category: Chen, Y]]
[[Category: Bryan P]]
[[Category: He, Y]]
[[Category: Chen Y]]
[[Category: Orban, J]]
[[Category: He Y]]
[[Category: Porter, L]]
[[Category: Orban J]]
[[Category: Ga]]
[[Category: Porter L]]
[[Category: Human serum albumin binding protein]]
[[Category: Maltose binding protein]]
[[Category: Mbp]]
[[Category: Protein binding]]

Latest revision as of 08:38, 7 February 2024

GA-79-MBP cs-rosetta structures

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