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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3jbm]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Orange-spotted_grouper_nervous_necrosis_virus Orange-spotted grouper nervous necrosis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JBM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JBM FirstGlance]. <br>
<table><tr><td colspan='2'>[[3jbm]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Orange-spotted_grouper_nervous_necrosis_virus Orange-spotted grouper nervous necrosis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JBM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JBM FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jbm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jbm OCA], [https://pdbe.org/3jbm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jbm RCSB], [https://www.ebi.ac.uk/pdbsum/3jbm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jbm ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jbm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jbm OCA], [https://pdbe.org/3jbm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jbm RCSB], [https://www.ebi.ac.uk/pdbsum/3jbm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jbm ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/A0A1L1QJU5_EPICO A0A1L1QJU5_EPICO]
Betanodavirus infection causes fatal disease of viral nervous necrosis in many cultured marine and freshwater fish worldwide and the virus-like particles (VLP) are effective vaccines against betanodavirus. But vaccine and viral vector designs of betanodavirus VLP based on their structures remain lacking. Here, the three-dimensional structure of orange-spotted grouper nervous necrosis virus (OGNNV) VLP (RBS) at 3.9 A reveals the organization of capsid proteins (CP). Based on the structural results, seven putative important sites were selected to genetically insert a 6x histidine (His)-tag for VLP formation screen, resulting in four His-tagged VLP (HV) at positions N-terminus, Ala220, Pro292 and C-terminus. The His-tags of N-terminal HV (NHV) were concealed inside virions while those of 220HV and C-terminal HV (CHV) were displayed at the outer surface. NHV, 220HV and CHV maintained the same cell entry ability as RBS in the Asian sea bass (SB) cell line, indicating that their similar surface structures can be recognized by the cellular entry receptor(s). For application of vaccine design, chromatography-purified CHV could provoke NNV-specific antibody responses as strong as those of RBS in a sea bass immunization assay. Furthermore, in carrying capacity assays, N-terminus and Ala220 can only carry short peptides and C-terminus can even accommodate large protein such as GFP to generate fluorescent VLP (CGV). For application of a viral vector, CGV could be real-time visualized to enter SB cells in invasion study. All the results confirmed that the C-terminus of CP is a suitable site to accommodate foreign peptides for vaccine design and viral vector development.
 
Structural analysis and insertion study reveal the ideal sites for surface displaying foreign peptides on a betanodavirus-like particle.,Xie J, Li K, Gao Y, Huang R, Lai Y, Shi Y, Yang S, Zhu G, Zhang Q, He J Vet Res. 2016 Jan 11;47:16. doi: 10.1186/s13567-015-0294-9. PMID:26754256<ref>PMID:26754256</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3jbm" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</SX>
</SX>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Orange-spotted grouper nervous necrosis virus]]
[[Category: Orange-spotted grouper nervous necrosis virus]]
[[Category: Gao, Y]]
[[Category: Gao Y]]
[[Category: He, J]]
[[Category: He J]]
[[Category: Huang, R]]
[[Category: Huang R]]
[[Category: Lai, Y]]
[[Category: Lai Y]]
[[Category: Li, K]]
[[Category: Li K]]
[[Category: Shi, Y]]
[[Category: Shi Y]]
[[Category: Xie, J]]
[[Category: Xie J]]
[[Category: Yang, S]]
[[Category: Yang S]]
[[Category: Zhang, Q]]
[[Category: Zhang Q]]
[[Category: Zhu, G]]
[[Category: Zhu G]]
[[Category: Betanodavirus]]
[[Category: Virus]]
[[Category: Virus-like particle]]

Latest revision as of 08:43, 7 February 2024

Electron cryo-microscopy of a virus-like particle of orange-spotted grouper nervous necrosis virus

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