1rym: Difference between revisions

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<StructureSection load='1rym' size='340' side='right'caption='[[1rym]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='1rym' size='340' side='right'caption='[[1rym]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1rym]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Maize Maize]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RYM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RYM FirstGlance]. <br>
<table><tr><td colspan='2'>[[1rym]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RYM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RYM FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ryb|1ryb]], [[1ryn|1ryn]]</div></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CRS2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rym FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rym OCA], [https://pdbe.org/1rym PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rym RCSB], [https://www.ebi.ac.uk/pdbsum/1rym PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rym ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rym FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rym OCA], [https://pdbe.org/1rym PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rym RCSB], [https://www.ebi.ac.uk/pdbsum/1rym PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rym ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/CRS2_MAIZE CRS2_MAIZE]] Required for the splicing of group IIB introns in chloroplasts. Forms complexes with either CAF1 or CAF2 which, in turn, interact with RNA and confer intron specificity of the splicing particles. Has no peptidyl-tRNA hydrolase activity.<ref>PMID:11179231</ref> <ref>PMID:9090875</ref> <ref>PMID:10481026</ref> <ref>PMID:12881426</ref>
[https://www.uniprot.org/uniprot/CRS2_MAIZE CRS2_MAIZE] Required for the splicing of group IIB introns in chloroplasts. Forms complexes with either CAF1 or CAF2 which, in turn, interact with RNA and confer intron specificity of the splicing particles. Has no peptidyl-tRNA hydrolase activity.<ref>PMID:11179231</ref> <ref>PMID:9090875</ref> <ref>PMID:10481026</ref> <ref>PMID:12881426</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rym ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rym ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Chloroplast RNA splicing 2 (CRS2) is a nuclear-encoded protein required for the splicing of nine group II introns in maize chloroplasts. CRS2 functions in the context of splicing complexes that include one of two CRS2-associated factors (CAF1 and CAF2). The CRS2-CAF1 and CRS2-CAF2 complexes are required for the splicing of different subsets of CRS2-dependent introns, and they bind tightly and specifically to their genetically defined intron targets in vivo. The CRS2 amino acid sequence is closely related to those of bacterial peptidyl-tRNA hydrolases (PTHs). To identify the structural differences between CRS2 and bacterial PTHs responsible for CRS2's gains of CAF binding and intron splicing functions, we determined the structure of CRS2 by X-ray crystallography. The fold of CRS2 is the same as that of Escherichia coli PTH, but CRS2 has two surfaces that differ from the corresponding surfaces in PTH. One of these is more hydrophobic in CRS2 than in PTH. Site-directed mutagenesis of this surface blocked CRS2-CAF complex formation, indicating that it is the CAF binding site. The CRS2 surface corresponding to the putative tRNA binding face of PTH is considerably more basic than in PTH, suggesting that CRS2 interacts with group II intron substrates via this surface. Both the sequence and the structural context of the amino acid residues essential for peptidyl-tRNA hydrolase activity are conserved in CRS2, yet expression of CRS2 is incapable of rescuing a pth(ts)E.coli strain.
Structural analysis of the group II intron splicing factor CRS2 yields insights into its protein and RNA interaction surfaces.,Ostheimer GJ, Hadjivassiliou H, Kloer DP, Barkan A, Matthews BW J Mol Biol. 2005 Jan 7;345(1):51-68. PMID:15567410<ref>PMID:15567410</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1rym" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Maize]]
[[Category: Zea mays]]
[[Category: Barkan, A]]
[[Category: Barkan A]]
[[Category: Matthews, B W]]
[[Category: Matthews BW]]
[[Category: Ostheimer, G J]]
[[Category: Ostheimer GJ]]
[[Category: Alpha-beta]]
[[Category: Translation]]

Latest revision as of 08:26, 14 February 2024

Structure of the Group II Intron Splicing Factor CRS2

1rym, resolution 1.80Å

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