2b98: Difference between revisions

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<StructureSection load='2b98' size='340' side='right'caption='[[2b98]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
<StructureSection load='2b98' size='340' side='right'caption='[[2b98]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2b98]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_43067 Atcc 43067]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B98 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B98 FirstGlance]. <br>
<table><tr><td colspan='2'>[[2b98]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B98 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B98 FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ribC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 ATCC 43067])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Riboflavin_synthase Riboflavin synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.9 2.5.1.9] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b98 OCA], [https://pdbe.org/2b98 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b98 RCSB], [https://www.ebi.ac.uk/pdbsum/2b98 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b98 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b98 OCA], [https://pdbe.org/2b98 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b98 RCSB], [https://www.ebi.ac.uk/pdbsum/2b98 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b98 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RISC_METJA RISC_METJA]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b98 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b98 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Whereas eubacterial and eukaryotic riboflavin synthases form homotrimers, archaeal riboflavin synthases from Methanocaldococcus jannaschii and Methanothermobacter thermoautrophicus are homopentamers with sequence similarity to the 6,7-dimethyl-8-ribityllumazine synthase catalyzing the penultimate step in riboflavin biosynthesis. Recently it could be shown that the complex dismutation reaction catalyzed by the pentameric M. jannaschii riboflavin synthase generates riboflavin with the same regiochemistry as observed for trimeric riboflavin synthases. Here we present crystal structures of the pentameric riboflavin synthase from M. jannaschii and its complex with the substrate analog inhibitor, 6,7-dioxo-8-ribityllumazine. The complex structure shows five active sites located between adjacent monomers of the pentamer. Each active site can accommodate two substrate analog molecules in anti-parallel orientation. The topology of the two bound ligands at the active site is well in line with the known stereochemistry of a pentacyclic adduct of 6,7-dimethyl-8-ribityllumazine that has been shown to serve as a kinetically competent intermediate. The pentacyclic intermediates of trimeric and pentameric riboflavin synthases are diastereomers.
Crystal structure of an archaeal pentameric riboflavin synthase in complex with a substrate analog inhibitor: stereochemical implications.,Ramsperger A, Augustin M, Schott AK, Gerhardt S, Krojer T, Eisenreich W, Illarionov B, Cushman M, Bacher A, Huber R, Fischer M J Biol Chem. 2006 Jan 13;281(2):1224-32. Epub 2005 Nov 4. PMID:16272154<ref>PMID:16272154</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2b98" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Atcc 43067]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Riboflavin synthase]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Augustin, M]]
[[Category: Augustin M]]
[[Category: Bacher, A]]
[[Category: Bacher A]]
[[Category: Cushman, M]]
[[Category: Cushman M]]
[[Category: Eisenreich, W]]
[[Category: Eisenreich W]]
[[Category: Fischer, M]]
[[Category: Fischer M]]
[[Category: Gerhardt, S]]
[[Category: Gerhardt S]]
[[Category: Huber, R]]
[[Category: Huber R]]
[[Category: Illarionov, B]]
[[Category: Illarionov B]]
[[Category: Krojer, T]]
[[Category: Krojer T]]
[[Category: Ramsperger, A]]
[[Category: Ramsperger A]]
[[Category: Schott, A K]]
[[Category: Schott AK]]
[[Category: Lumazine riboflavin]]
[[Category: Transferase]]
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