2fqm: Difference between revisions

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<StructureSection load='2fqm' size='340' side='right'caption='[[2fqm]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
<StructureSection load='2fqm' size='340' side='right'caption='[[2fqm]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2fqm]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Vsiv Vsiv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FQM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FQM FirstGlance]. <br>
<table><tr><td colspan='2'>[[2fqm]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Vesicular_stomatitis_Indiana_virus Vesicular stomatitis Indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FQM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FQM FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">P ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11277 VSIV])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fqm OCA], [https://pdbe.org/2fqm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fqm RCSB], [https://www.ebi.ac.uk/pdbsum/2fqm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fqm ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fqm OCA], [https://pdbe.org/2fqm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fqm RCSB], [https://www.ebi.ac.uk/pdbsum/2fqm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fqm ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/PHOSP_VSIVM PHOSP_VSIVM]] Essential component of the RNA polymerase transcription and replication complex. Binds the viral ribonucleocapsid and positions the L polymerase on the template. May act as a chaperone for newly synthesized free N protein, so-called N(0). Plays a role in virion assembly (By similarity).  
[https://www.uniprot.org/uniprot/PHOSP_VSIVM PHOSP_VSIVM] Essential component of the RNA polymerase transcription and replication complex. Binds the viral ribonucleocapsid and positions the L polymerase on the template. May act as a chaperone for newly synthesized free N protein, so-called N(0). Plays a role in virion assembly (By similarity).
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fqm ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fqm ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In the replication cycle of nonsegmented negative-strand RNA viruses, the viral RNA-dependent RNA polymerase (L) recognizes a nucleoprotein (N)-enwrapped RNA template during the RNA polymerase reaction. The viral phosphoprotein (P) is a polymerase cofactor essential for this recognition. We report here the 2.3-angstroms-resolution crystal structure of the central domain (residues 107 to 177) of P from vesicular stomatitis virus. The fold of this domain consists of a beta hairpin, an alpha helix, and another beta hairpin. The alpha helix provides the stabilizing force for forming a homodimer, while the two beta hairpins add additional stabilization by forming a four-stranded beta sheet through domain swapping between two molecules. This central dimer positions the N- and C-terminal domains of P to interact with the N and L proteins, allowing the L protein to specifically recognize the nucleocapsid-RNA template and to progress along the template while concomitantly assembling N with nascent RNA. The interdimer interactions observed in the noncrystallographic packing may offer insight into the mechanism of the RNA polymerase processive reaction along the viral nucleocapsid-RNA template.
Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus.,Ding H, Green TJ, Lu S, Luo M J Virol. 2006 Mar;80(6):2808-14. PMID:16501089<ref>PMID:16501089</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2fqm" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Vsiv]]
[[Category: Vesicular stomatitis Indiana virus]]
[[Category: Ding, H]]
[[Category: Ding H]]
[[Category: Green, T J]]
[[Category: Green TJ]]
[[Category: Lu, S]]
[[Category: Lu S]]
[[Category: Luo, M]]
[[Category: Luo M]]
[[Category: Cofactor]]
[[Category: Negative strand rna virus]]
[[Category: Polymerase]]
[[Category: Replication]]
[[Category: Viral protein]]

Latest revision as of 09:24, 14 February 2024

Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus

2fqm, resolution 2.30Å

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