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<StructureSection load='7ute' size='340' side='right'caption='[[7ute]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
<StructureSection load='7ute' size='340' side='right'caption='[[7ute]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7ute]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UTE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UTE FirstGlance]. <br>
<table><tr><td colspan='2'>[[7ute]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeropyrum_pernix Aeropyrum pernix]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UTE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UTE FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ute FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ute OCA], [https://pdbe.org/7ute PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ute RCSB], [https://www.ebi.ac.uk/pdbsum/7ute PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ute ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ute FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ute OCA], [https://pdbe.org/7ute PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ute RCSB], [https://www.ebi.ac.uk/pdbsum/7ute PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ute ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q9YFF4_AERPE Q9YFF4_AERPE]
Biocatalytic carbene transfer from diazo compounds is a versatile strategy in asymmetric synthesis. However, the limited pool of stable diazo compounds constrains the variety of accessible products. To overcome this restriction, we have engineered variants of Aeropyrum pernix protoglobin (ApePgb) that use diazirines as carbene precursors. While the enhanced stability of diazirines relative to their diazo isomers enables access to a diverse array of carbenes, they have previously resisted catalytic activation. Our engineered ApePgb variants represent the first example of catalysts for selective carbene transfer from these species at room temperature. The structure of an ApePgb variant, determined by microcrystal electron diffraction (MicroED), reveals that evolution has enhanced access to the heme active site to facilitate this new-to-nature catalysis. Using readily prepared aryl diazirines as model substrates, we demonstrate the application of these highly stable carbene precursors in biocatalytic cyclopropanation, N-H insertion, and Si-H insertion reactions.
 
Biocatalytic Carbene Transfer Using Diazirines.,Porter NJ, Danelius E, Gonen T, Arnold FH J Am Chem Soc. 2022 May 13. doi: 10.1021/jacs.2c02723. PMID:35561334<ref>PMID:35561334</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7ute" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Aeropyrum pernix]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Danelius, E]]
[[Category: Danelius E]]
[[Category: Gonen, T]]
[[Category: Gonen T]]
[[Category: Unge, J T]]
[[Category: Unge JT]]
[[Category: Heme]]
[[Category: Metal binding protein]]
[[Category: Microed]]
[[Category: Protoglobin]]

Revision as of 09:39, 14 February 2024

MicroED structure of Aeropyrum pernix protoglobin mutant

7ute, resolution 2.10Å

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