3fi1: Difference between revisions

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<StructureSection load='3fi1' size='340' side='right'caption='[[3fi1]], [[Resolution|resolution]] 7.00&Aring;' scene=''>
<StructureSection load='3fi1' size='340' side='right'caption='[[3fi1]], [[Resolution|resolution]] 7.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3fi1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FI1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FI1 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3fi1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FI1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FI1 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fi1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fi1 OCA], [https://pdbe.org/3fi1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fi1 RCSB], [https://www.ebi.ac.uk/pdbsum/3fi1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fi1 ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fi1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fi1 OCA], [https://pdbe.org/3fi1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fi1 RCSB], [https://www.ebi.ac.uk/pdbsum/3fi1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fi1 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/NHAA_ECOLI NHAA_ECOLI]] Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons. Catalyzes the exchange of 2 H(+) per Na(+). Can mediate sodium uptake when a transmembrane pH gradient is applied. Active at alkaline pH. Activity is strongly down-regulated below pH 6.5.<ref>PMID:1645730</ref>
[https://www.uniprot.org/uniprot/NHAA_ECOLI NHAA_ECOLI] Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons. Catalyzes the exchange of 2 H(+) per Na(+). Can mediate sodium uptake when a transmembrane pH gradient is applied. Active at alkaline pH. Activity is strongly down-regulated below pH 6.5.<ref>PMID:1645730</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fi1 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fi1 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
NhaA, the main sodium-proton exchanger in the inner membrane of Escherichia coli, regulates the cytosolic concentrations of H and Na. It is inactive at acidic pH, becomes active between pH 6 and pH 7, and reaches maximum activity at pH 8. By cryo-electron microscopy of two-dimensional crystals grown at pH 4 and incubated at higher pH, we identified two sequential conformational changes in the protein in response to pH or substrate ions. The first change is induced by a rise in pH from 6 to 7 and marks the transition from the inactive state to the pH-activated state. pH activation, which precedes the ion-induced conformational change, is accompanied by an overall expansion of the NhaA monomer and a local ordering of the N-terminus. The second conformational change is induced by the substrate ions Na and Li at pH above 7 and involves a 7-A displacement of helix IVp. This movement would cause a charge imbalance at the ion-binding site that may trigger the release of the substrate ion and open a periplasmic exit channel.
Conformations of NhaA, the Na/H exchanger from Escherichia coli, in the pH-activated and ion-translocating states.,Appel M, Hizlan D, Vinothkumar KR, Ziegler C, Kuhlbrandt W J Mol Biol. 2009 Feb 20;386(2):351-65. Epub 2008 Dec 25. PMID:19135453<ref>PMID:19135453</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3fi1" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Escherichia coli k-12]]
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Appel, M]]
[[Category: Appel M]]
[[Category: Hizlan, D]]
[[Category: Hizlan D]]
[[Category: Kuehlbrandt, W]]
[[Category: Kuehlbrandt W]]
[[Category: Vinothkumar, K R]]
[[Category: Vinothkumar KR]]
[[Category: Ziegler, C]]
[[Category: Ziegler C]]
[[Category: Antiport]]
[[Category: Cell inner membrane]]
[[Category: Cell membrane]]
[[Category: Ion transport]]
[[Category: Membrane]]
[[Category: Membrane protein]]
[[Category: Membrane protein sodium proton antiporter]]
[[Category: Sodium transport]]
[[Category: Transmembrane]]
[[Category: Transport]]
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