3h4r: Difference between revisions

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<StructureSection load='3h4r' size='340' side='right'caption='[[3h4r]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
<StructureSection load='3h4r' size='340' side='right'caption='[[3h4r]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3h4r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H4R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3H4R FirstGlance]. <br>
<table><tr><td colspan='2'>[[3h4r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H4R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3H4R FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b1350, JW1344, recE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3h4r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h4r OCA], [https://pdbe.org/3h4r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3h4r RCSB], [https://www.ebi.ac.uk/pdbsum/3h4r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3h4r ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3h4r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h4r OCA], [https://pdbe.org/3h4r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3h4r RCSB], [https://www.ebi.ac.uk/pdbsum/3h4r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3h4r ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/RECE_ECOLI RECE_ECOLI]] Is involved in the RecE pathway of recombination. Has a strong preference for linear duplex substrate DNA and appears to be unable to initiate degradation from single-stranded breaks in DNA.  
[https://www.uniprot.org/uniprot/RECE_ECOLI RECE_ECOLI] Is involved in the RecE pathway of recombination. Has a strong preference for linear duplex substrate DNA and appears to be unable to initiate degradation from single-stranded breaks in DNA.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3h4r ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3h4r ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Escherichia coli RecE protein is part of the classical RecET recombination system that has recently been used in powerful new methods for genetic engineering. RecE binds to free double-stranded DNA (dsDNA) ends and processively digests the 5'-ended strand to form 5'-mononucleotides and a 3'-overhang that is a substrate for single strand annealing promoted by RecT. Here, we report the crystal structure of the C-terminal nuclease domain of RecE at 2.8 A resolution. RecE forms a toroidal tetramer with a central tapered channel that is wide enough to bind dsDNA at one end, but is partially plugged at the other end by the C-terminal segment of the protein. Four narrow tunnels, one within each subunit of the tetramer, lead from the central channel to the four active sites, which lie about 15 A from the channel. The structure, combined with mutational studies, suggests a mechanism in which dsDNA enters through the open end of the central channel, the 5'-ended strand passes through a tunnel to access one of the four active sites, and the 3'-ended strand passes through the plugged end of the channel at the back of the tetramer.
Crystal structure of E. coli RecE protein reveals a toroidal tetramer for processing double-stranded DNA breaks.,Zhang J, Xing X, Herr AB, Bell CE Structure. 2009 May 13;17(5):690-702. PMID:19446525<ref>PMID:19446525</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3h4r" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Exonuclease 3D structures|Exonuclease 3D structures]]
*[[Exonuclease 3D structures|Exonuclease 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Ecoli]]
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Bell, C E]]
[[Category: Bell CE]]
[[Category: Zhang, J]]
[[Category: Zhang J]]
[[Category: Exonuclease]]
[[Category: Hydrolase]]
[[Category: Nuclease]]
[[Category: Recombination]]
Retrieved from "https://proteopedia.org/3h4r"