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<StructureSection load='3hef' size='340' side='right'caption='[[3hef]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
<StructureSection load='3hef' size='340' side='right'caption='[[3hef]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3hef]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpsfv Bpsfv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HEF FirstGlance]. <br>
<table><tr><td colspan='2'>[[3hef]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_virus_Sf6 Shigella virus Sf6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HEF FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gp1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10761 BPSFV])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hef OCA], [https://pdbe.org/3hef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hef RCSB], [https://www.ebi.ac.uk/pdbsum/3hef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hef ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hef OCA], [https://pdbe.org/3hef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hef RCSB], [https://www.ebi.ac.uk/pdbsum/3hef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hef ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q716H4_BPSFV Q716H4_BPSFV]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hef ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hef ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In herpesviruses and many bacterial viruses, genome-packaging is a precisely mediated process fulfilled by a virally encoded molecular machine called terminase that consists of two protein components: A DNA-recognition component that defines the specificity for packaged DNA, and a catalytic component that provides energy for the packaging reaction by hydrolyzing ATP. The terminase docks onto the portal protein complex embedded in a single vertex of a preformed viral protein shell called procapsid, and pumps the viral DNA into the procapsid through a conduit formed by the portal. Here we report the 1.65 A resolution structure of the DNA-recognition component gp1 of the Shigella bacteriophage Sf6 genome-packaging machine. The structure reveals a ring-like octamer formed by interweaved protein monomers with a highly extended fold, embracing a tunnel through which DNA may be translocated. The N-terminal DNA-binding domains form the peripheral appendages surrounding the octamer. The central domain contributes to oligomerization through interactions of bundled helices. The C-terminal domain forms a barrel with parallel beta-strands. The structure reveals a common scheme for oligomerization of terminase DNA-recognition components, and provides insights into the role of gp1 in formation of the packaging-competent terminase complex and assembly of the terminase with the portal, in which ring-like protein oligomers stack together to form a continuous channel for viral DNA translocation.
Crystal structure of the DNA-recognition component of the bacterial virus Sf6 genome-packaging machine.,Zhao H, Finch CJ, Sequeira RD, Johnson BA, Johnson JE, Casjens SR, Tang L Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):1971-6. Epub 2010 Jan 19. PMID:20133842<ref>PMID:20133842</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3hef" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Terminase|Terminase]]
*[[Terminase 3D Structures|Terminase 3D Structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bpsfv]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Tang, L]]
[[Category: Shigella virus Sf6]]
[[Category: Zhao, H]]
[[Category: Tang L]]
[[Category: Bacteriophage sf6]]
[[Category: Zhao H]]
[[Category: Dna packaging]]
[[Category: Dna recognition]]
[[Category: Gp1 channel]]
[[Category: Gp1 octameric assembly]]
[[Category: Terminase small subunit gp1]]
[[Category: Viral protein]]
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