3htk: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
Line 3: Line 3:
<StructureSection load='3htk' size='340' side='right'caption='[[3htk]], [[Resolution|resolution]] 2.31&Aring;' scene=''>
<StructureSection load='3htk' size='340' side='right'caption='[[3htk]], [[Resolution|resolution]] 2.31&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3htk]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HTK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HTK FirstGlance]. <br>
<table><tr><td colspan='2'>[[3htk]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HTK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HTK FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.31&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SMC5, YOL034W ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), MMS21, NSE2, YEL019C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3htk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3htk OCA], [https://pdbe.org/3htk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3htk RCSB], [https://www.ebi.ac.uk/pdbsum/3htk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3htk ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3htk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3htk OCA], [https://pdbe.org/3htk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3htk RCSB], [https://www.ebi.ac.uk/pdbsum/3htk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3htk ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/SMC5_YEAST SMC5_YEAST]] Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks. [[https://www.uniprot.org/uniprot/NSE2_YEAST NSE2_YEAST]] Acts as an E3 ligase mediating SUMO/Smt3 attachment to SMC5 and YKU70. Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks.<ref>PMID:15738391</ref> 
[https://www.uniprot.org/uniprot/SMC5_YEAST SMC5_YEAST] Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Line 20: Line 20:
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3htk ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3htk ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Smc5/6 complex is an evolutionarily conserved chromosomal ATPase required for cell growth and DNA repair. Its Mms21 subunit supports both functions by docking to the arm region of Smc5 and providing SUMO ligase activity. Here, we report the crystal structure of Mms21 in complex with the Smc5 arm. Our structure revealed two distinct structural and functional domains of the Smc5-bound Mms21: its N-terminal half is dedicated to Smc5 binding by forming a helix bundle with a coiled-coil structure of Smc5; its C-terminal half includes the SUMO ligase domain, which adopts a new type of RING E3 structure. Mutagenesis and structural analyses showed that the Mms21-Smc5 interface is required for cell growth and resistance to DNA damage, while the unique Mms21 RING domain confers specificity to the SUMO E2-E3 interaction. Through structure-based dissection of Mms21 functions, our studies establish a framework for understanding its roles in the Smc5/6 complex.
Structural and functional insights into the roles of the Mms21 subunit of the Smc5/6 complex.,Duan X, Sarangi P, Liu X, Rangi GK, Zhao X, Ye H Mol Cell. 2009 Sep 11;35(5):657-68. PMID:19748359<ref>PMID:19748359</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3htk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Atcc 18824]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Duan, X]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Liu, X]]
[[Category: Duan X]]
[[Category: Rangi, G K]]
[[Category: Liu X]]
[[Category: Sarangi, P]]
[[Category: Rangi GK]]
[[Category: Ye, H]]
[[Category: Sarangi P]]
[[Category: Zhao, X]]
[[Category: Ye H]]
[[Category: Atp-binding]]
[[Category: Zhao X]]
[[Category: Chromosomal protein]]
[[Category: Coiled coil]]
[[Category: Cytoplasm]]
[[Category: Dna damage]]
[[Category: Dna recombination]]
[[Category: Dna repair]]
[[Category: Ligase]]
[[Category: Metal-binding]]
[[Category: Nucleotide-binding]]
[[Category: Nucleus]]
[[Category: Recombination-replication-ligase complex]]
[[Category: Ring]]
[[Category: Spl-ring]]
[[Category: Sumo e3 ligase]]
[[Category: Ubl conjugation]]
[[Category: Ubl conjugation pathway]]
[[Category: Zinc]]
[[Category: Zinc-finger]]

Latest revision as of 10:01, 21 February 2024

Crystal structure of Mms21 and Smc5 complex

3htk, resolution 2.31Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=3htk&oldid=4065388"