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==Crystal structure of human mitochondrial mTERF in complex with a 15-mer DNA encompassing the tRNALeu(UUR) binding sequence==
==Crystal structure of human mitochondrial mTERF in complex with a 15-mer DNA encompassing the tRNALeu(UUR) binding sequence==
<StructureSection load='3n6s' size='340' side='right' caption='[[3n6s]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
<StructureSection load='3n6s' size='340' side='right'caption='[[3n6s]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3n6s]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N6S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N6S FirstGlance]. <br>
<table><tr><td colspan='2'>[[3n6s]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N6S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N6S FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3n7q|3n7q]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MTERF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n6s OCA], [https://pdbe.org/3n6s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n6s RCSB], [https://www.ebi.ac.uk/pdbsum/3n6s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n6s ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n6s OCA], [http://pdbe.org/3n6s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3n6s RCSB], [http://www.ebi.ac.uk/pdbsum/3n6s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3n6s ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/MTERF_HUMAN MTERF_HUMAN]] Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Probably requires one or more components for termination activity.<ref>PMID:20550934</ref>
[https://www.uniprot.org/uniprot/MTEF1_HUMAN MTEF1_HUMAN] Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Transcription termination activity appears to be polarized with highest specificity for transcripts initiated on the light strand.<ref>PMID:20550934</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3n6s ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3n6s ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The regulation of mitochondrial DNA (mtDNA) processes is slowly being characterized at a structural level. We present here crystal structures of human mitochondrial regulator mTERF, a transcription termination factor also implicated in replication pausing, in complex with double-stranded DNA oligonucleotides containing the tRNA(Leu)(UUR) gene sequence. mTERF comprises nine left-handed helical tandem repeats that form a left-handed superhelix, the Zurdo domain.
Human mitochondrial mTERF wraps around DNA through a left-handed superhelical tandem repeat.,Jimenez-Menendez N, Fernandez-Millan P, Rubio-Cosials A, Arnan C, Montoya J, Jacobs HT, Bernado P, Coll M, Uson I, Sola M Nat Struct Mol Biol. 2010 Jul;17(7):891-3. Epub 2010 Jun 13. PMID:20543826<ref>PMID:20543826</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3n6s" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Human]]
[[Category: Homo sapiens]]
[[Category: Arnan, C]]
[[Category: Large Structures]]
[[Category: Bernado, P]]
[[Category: Arnan C]]
[[Category: Coll, M]]
[[Category: Bernado P]]
[[Category: Fernandez-Millan, P]]
[[Category: Coll M]]
[[Category: Jacobs, H T]]
[[Category: Fernandez-Millan P]]
[[Category: Jimenez-Menendez, N]]
[[Category: Jacobs HT]]
[[Category: Montoya, J]]
[[Category: Jimenez-Menendez N]]
[[Category: Rubio-Cosials, A]]
[[Category: Montoya J]]
[[Category: Sola, M]]
[[Category: Rubio-Cosials A]]
[[Category: Uson, I]]
[[Category: Sola M]]
[[Category: Left-handed helical tandem repeat]]
[[Category: Uson I]]
[[Category: Mitochondrial replication pausing-dna complex]]
[[Category: Mitochondrial transcription termination factor-dna complex]]
[[Category: Protein-dna complex]]
[[Category: Replication-dna complex]]
[[Category: Transcription]]
Retrieved from "https://proteopedia.org/3n6s"