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==Crystal structure of extended Dimerization module of RNA polymerase I subcomplex A49/A34.5==
==Crystal structure of extended Dimerization module of RNA polymerase I subcomplex A49/A34.5==
<StructureSection load='3nff' size='340' side='right' caption='[[3nff]], [[Resolution|resolution]] 3.24&Aring;' scene=''>
<StructureSection load='3nff' size='340' side='right'caption='[[3nff]], [[Resolution|resolution]] 3.24&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3nff]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_2001 Atcc 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NFF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NFF FirstGlance]. <br>
<table><tr><td colspan='2'>[[3nff]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_glabrata Candida glabrata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NFF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NFF FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CAGL0J07766g, rpa49 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5478 ATCC 2001]), CAGL0I06006g, rpa34 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5478 ATCC 2001])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.24&#8491;</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nff OCA], [https://pdbe.org/3nff PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nff RCSB], [https://www.ebi.ac.uk/pdbsum/3nff PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nff ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nff OCA], [http://pdbe.org/3nff PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nff RCSB], [http://www.ebi.ac.uk/pdbsum/3nff PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nff ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q6FNZ9_CANGA Q6FNZ9_CANGA]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3nff ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3nff ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The eukaryotic RNA polymerases Pol I, II, and III use different promoters to transcribe different classes of genes. Promoter usage relies on initiation factors, including TFIIF and TFIIE, in the case of Pol II. Here, we show that the Pol I-specific subunits A49 and A34.5 form a subcomplex that binds DNA and is related to TFIIF and TFIIE. The N-terminal regions of A49 and A34.5 form a dimerization module that stimulates polymerase-intrinsic RNA cleavage and has a fold that resembles the TFIIF core. The C-terminal region of A49 forms a "tandem winged helix" (tWH) domain that binds DNA with a preference for the upstream promoter nontemplate strand and is predicted in TFIIE. Similar domains are predicted in Pol III-specific subunits. Thus, Pol I/III subunits that have no counterparts in Pol II are evolutionarily related to Pol II initiation factors and may have evolved to mediate promoter specificity and transcription processivity.
RNA polymerase I contains a TFIIF-related DNA-binding subcomplex.,Geiger SR, Lorenzen K, Schreieck A, Hanecker P, Kostrewa D, Heck AJ, Cramer P Mol Cell. 2010 Aug 27;39(4):583-94. PMID:20797630<ref>PMID:20797630</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3nff" style="background-color:#fffaf0;"></div>
==See Also==
*[[RNA polymerase|RNA polymerase]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Atcc 2001]]
[[Category: Large Structures]]
[[Category: DNA-directed RNA polymerase]]
[[Category: Cramer P]]
[[Category: Cramer, P]]
[[Category: Geiger SR]]
[[Category: Geiger, S R]]
[[Category: Hanecker P]]
[[Category: Hanecker, P]]
[[Category: Heck AJR]]
[[Category: Heck, A J.R]]
[[Category: Kostrewa D]]
[[Category: Kostrewa, D]]
[[Category: Lorenzen K]]
[[Category: Lorenzen, K]]
[[Category: Schreieck A]]
[[Category: Schreieck, A]]
[[Category: Dimerization]]
[[Category: Rna polymerase]]
[[Category: Transcription]]
[[Category: Triple barrel]]
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