3r4f: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3r4f]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R4F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R4F FirstGlance]. <br>
<table><tr><td colspan='2'>[[3r4f]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R4F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R4F FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r4f OCA], [https://pdbe.org/3r4f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r4f RCSB], [https://www.ebi.ac.uk/pdbsum/3r4f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r4f ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r4f OCA], [https://pdbe.org/3r4f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r4f RCSB], [https://www.ebi.ac.uk/pdbsum/3r4f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r4f ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Prohead RNA (pRNA) is an essential component in the assembly and operation of the powerful bacteriophage 29 DNA packaging motor. The pRNA forms a multimeric ring via intermolecular base-pairing interactions between protomers that serves to guide the assembly of the ring ATPase that drives DNA packaging. Here we report the quaternary structure of this rare multimeric RNA at 3.5 A resolution, crystallized as tetrameric rings. Strong quaternary interactions and the inherent flexibility helped rationalize how free pRNA is able to adopt multiple oligomerization states in solution. These characteristics also allowed excellent fitting of the crystallographic pRNA protomers into previous prohead/pRNA cryo-EM reconstructions, supporting the presence of a pentameric, but not hexameric, pRNA ring in the context of the DNA packaging motor. The pentameric pRNA ring anchors itself directly to the phage prohead by interacting specifically with the fivefold symmetric capsid structures that surround the head-tail connector portal. From these contacts, five RNA superhelices project from the pRNA ring, where they serve as scaffolds for binding and assembly of the ring ATPase, and possibly mediate communication between motor components. Construction of structure-based designer pRNAs with little sequence similarity to the wild-type pRNA were shown to fully support the packaging of 29 DNA.
Structure and assembly of the essential RNA ring component of a viral DNA packaging motor.,Ding F, Lu C, Zhao W, Rajashankar KR, Anderson DL, Jardine PJ, Grimes S, Ke A Proc Natl Acad Sci U S A. 2011 Apr 6. PMID:21471452<ref>PMID:21471452</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3r4f" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Anderson, D L]]
[[Category: Anderson DL]]
[[Category: Ding, F]]
[[Category: Ding F]]
[[Category: Grimes, S]]
[[Category: Grimes S]]
[[Category: Jardine, P J]]
[[Category: Jardine PJ]]
[[Category: Ke, A]]
[[Category: Ke A]]
[[Category: Lu, C]]
[[Category: Lu C]]
[[Category: Rajashankar, K R]]
[[Category: Rajashankar KR]]
[[Category: Zhano, W]]
[[Category: Zhano W]]
[[Category: Motor]]
[[Category: Prohead rna]]
[[Category: Rna]]
[[Category: Viral packaging]]
Retrieved from "https://proteopedia.org/3r4f"