8qdo: Difference between revisions

Latest revision as of 09:39, 1 March 2024

Crystal structure of the tegument protein UL82 (pp71) from Human Cytomegalovirus

Structural highlights

8qdo is a 2 chain structure with sequence from Human betaherpesvirus 5. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.7Å
Ligands:	PG4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PP71_HCMVA Stimulates viral immediate-early (IE) transcription. Plays a role in the inhibition of the host innate repsonse by targeting STING1 and thus the cGAS-STING pathway (PubMed:28132838, PubMed:32581105). Counteracts also host DAXX-mediated repression of viral transcription. Displaces a DAXX-binding protein, ATRX, from nuclear domain 10 sites (ND10) shortly after infection (PubMed:18922870). Increases the basal level of SUMOylated DAXX in infected cells (PubMed:19369322). Stimulates quiescent cells to re-enter the cell cycle, proceed through G1 and enter the S phase (PubMed:12610120). Interacts with hypophosphorylated forms of RB1 and induces their degradation by the proteasome without involving ubiquitin conjugation (PubMed:12626766).^[1] ^[2] ^[3] ^[4] ^[5] ^[6]

References

↑ Kalejta RF, Shenk T. The human cytomegalovirus UL82 gene product (pp71) accelerates progression through the G1 phase of the cell cycle. J Virol. 2003 Mar;77(6):3451-9. PMID:12610120 doi:10.1128/jvi.77.6.3451-3459.2003
↑ Kalejta RF, Shenk T. Proteasome-dependent, ubiquitin-independent degradation of the Rb family of tumor suppressors by the human cytomegalovirus pp71 protein. Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3263-8. PMID:12626766 doi:10.1073/pnas.0538058100
↑ Lukashchuk V, McFarlane S, Everett RD, Preston CM. Human cytomegalovirus protein pp71 displaces the chromatin-associated factor ATRX from nuclear domain 10 at early stages of infection. J Virol. 2008 Dec;82(24):12543-54. PMID:18922870 doi:10.1128/JVI.01215-08
↑ Hwang J, Kalejta RF. Human cytomegalovirus protein pp71 induces Daxx SUMOylation. J Virol. 2009 Jul;83(13):6591-8. PMID:19369322 doi:10.1128/JVI.02639-08
↑ Fu YZ, Su S, Gao YQ, Wang PP, Huang ZF, Hu MM, Luo WW, Li S, Luo MH, Wang YY, Shu HB. Human Cytomegalovirus Tegument Protein UL82 Inhibits STING-Mediated Signaling to Evade Antiviral Immunity. Cell Host Microbe. 2017 Feb 8;21(2):231-243. PMID:28132838 doi:10.1016/j.chom.2017.01.001
↑ Nukui M, Roche KL, Jia J, Fox PL, Murphy EA. Protein S-Nitrosylation of Human Cytomegalovirus pp71 Inhibits Its Ability To Limit STING Antiviral Responses. J Virol. 2020 Aug 17;94(17):e00033-20. PMID:32581105 doi:10.1128/JVI.00033-20

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OCA

[1] Kalejta RF, Shenk T. The human cytomegalovirus UL82 gene product (pp71) accelerates progression through the G1 phase of the cell cycle. J Virol. 2003 Mar;77(6):3451-9. PMID:12610120 doi:10.1128/jvi.77.6.3451-3459.2003

[2] Kalejta RF, Shenk T. Proteasome-dependent, ubiquitin-independent degradation of the Rb family of tumor suppressors by the human cytomegalovirus pp71 protein. Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3263-8. PMID:12626766 doi:10.1073/pnas.0538058100

[3] Lukashchuk V, McFarlane S, Everett RD, Preston CM. Human cytomegalovirus protein pp71 displaces the chromatin-associated factor ATRX from nuclear domain 10 at early stages of infection. J Virol. 2008 Dec;82(24):12543-54. PMID:18922870 doi:10.1128/JVI.01215-08

[4] Hwang J, Kalejta RF. Human cytomegalovirus protein pp71 induces Daxx SUMOylation. J Virol. 2009 Jul;83(13):6591-8. PMID:19369322 doi:10.1128/JVI.02639-08

[5] Fu YZ, Su S, Gao YQ, Wang PP, Huang ZF, Hu MM, Luo WW, Li S, Luo MH, Wang YY, Shu HB. Human Cytomegalovirus Tegument Protein UL82 Inhibits STING-Mediated Signaling to Evade Antiviral Immunity. Cell Host Microbe. 2017 Feb 8;21(2):231-243. PMID:28132838 doi:10.1016/j.chom.2017.01.001

[6] Nukui M, Roche KL, Jia J, Fox PL, Murphy EA. Protein S-Nitrosylation of Human Cytomegalovirus pp71 Inhibits Its Ability To Limit STING Antiviral Responses. J Virol. 2020 Aug 17;94(17):e00033-20. PMID:32581105 doi:10.1128/JVI.00033-20

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 8qdo is ON HOLD  until Paper Publication
+==Crystal structure of the tegument protein UL82 (pp71) from Human Cytomegalovirus==
+<StructureSection load='8qdo' size='340' side='right'caption='[[8qdo]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8qdo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_betaherpesvirus_5 Human betaherpesvirus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8QDO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8QDO FirstGlance]. <br>
-Description:
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8qdo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8qdo OCA], [https://pdbe.org/8qdo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8qdo RCSB], [https://www.ebi.ac.uk/pdbsum/8qdo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8qdo ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PP71_HCMVA PP71_HCMVA] Stimulates viral immediate-early (IE) transcription. Plays a role in the inhibition of the host innate repsonse by targeting STING1 and thus the cGAS-STING pathway (PubMed:28132838, PubMed:32581105). Counteracts also host DAXX-mediated repression of viral transcription. Displaces a DAXX-binding protein, ATRX, from nuclear domain 10 sites (ND10) shortly after infection (PubMed:18922870). Increases the basal level of SUMOylated DAXX in infected cells (PubMed:19369322). Stimulates quiescent cells to re-enter the cell cycle, proceed through G1 and enter the S phase (PubMed:12610120). Interacts with hypophosphorylated forms of RB1 and induces their degradation by the proteasome without involving ubiquitin conjugation (PubMed:12626766).<ref>PMID:12610120</ref> <ref>PMID:12626766</ref> <ref>PMID:18922870</ref> <ref>PMID:19369322</ref> <ref>PMID:28132838</ref> <ref>PMID:32581105</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Human betaherpesvirus 5]]
+[[Category: Large Structures]]
+[[Category: Bresch IP]]
+[[Category: Eberhage J]]
+[[Category: Eschenburg S]]
+[[Category: Reubold TF]]

8qdo: Difference between revisions

Latest revision as of 09:39, 1 March 2024

Crystal structure of the tegument protein UL82 (pp71) from Human Cytomegalovirus

Structural highlights

Function

References

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