4v7f: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4v7f]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3j64 3j64], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3j65 3j65] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3j66 3j66]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V7F FirstGlance]. <br>
<table><tr><td colspan='2'>[[4v7f]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3j64 3j64], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3j65 3j65] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3j66 3j66]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V7F FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v7f OCA], [https://pdbe.org/4v7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v7f RCSB], [https://www.ebi.ac.uk/pdbsum/4v7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v7f ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v7f OCA], [https://pdbe.org/4v7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v7f RCSB], [https://www.ebi.ac.uk/pdbsum/4v7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v7f ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[https://www.uniprot.org/uniprot/RL1A_YEAST RL1A_YEAST]  
[https://www.uniprot.org/uniprot/RL1A_YEAST RL1A_YEAST]  
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== Publication Abstract from PubMed ==
During eukaryotic ribosome biogenesis, nascent ribosomal RNA (rRNA) forms pre-ribosomal particles containing ribosomal proteins and assembly factors. Subsequently, these immature rRNAs are processed and remodelled. Little is known about the premature assembly states of rRNAs and their structural rearrangement during ribosome biogenesis. Using cryo-EM we characterize a pre-60S particle, where the 5S rRNA and its associated ribosomal proteins L18 and L5 (5S ribonucleoprotein (RNP)) are rotated by almost 180 degrees when compared with the mature subunit. Consequently, neighbouring 25S rRNA helices that protrude from the base of the central protuberance are deformed. This altered topology is stabilized by nearby assembly factors (Rsa4 and Nog1), which were identified by fitting their three-dimensional structures into the cryo-EM density. We suggest that the 5S RNP performs a semicircular movement during 60S biogenesis to adopt its final position, fulfilling a chaperone-like function in guiding the flanking 25S rRNA helices of the central protuberance to their final topology.


60S ribosome biogenesis requires rotation of the 5S ribonucleoprotein particle.,Leidig C, Thoms M, Holdermann I, Bradatsch B, Berninghausen O, Bange G, Sinning I, Hurt E, Beckmann R Nat Commun. 2014 Mar 24;5:3491. doi: 10.1038/ncomms4491. PMID:24662372<ref>PMID:24662372</ref>
==See Also==
 
*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
*[[Ribosome biogenesis protein 3D structures|Ribosome biogenesis protein 3D structures]]
</div>
<div class="pdbe-citations 4v7f" style="background-color:#fffaf0;"></div>
== References ==
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