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==Structure of IMP dehydrogenase from Ashbya gossypii bound to ATP and GDP==
==Structure of IMP dehydrogenase from Ashbya gossypii bound to ATP and GDP==
<StructureSection load='5tc3' size='340' side='right' caption='[[5tc3]], [[Resolution|resolution]] 2.46&Aring;' scene=''>
<StructureSection load='5tc3' size='340' side='right'caption='[[5tc3]], [[Resolution|resolution]] 2.46&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5tc3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ashgo Ashgo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TC3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TC3 FirstGlance]. <br>
<table><tr><td colspan='2'>[[5tc3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Eremothecium_gossypii_ATCC_10895 Eremothecium gossypii ATCC 10895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TC3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TC3 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.462&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AGOS_AER117W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284811 ASHGO])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/IMP_dehydrogenase IMP dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.205 1.1.1.205] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tc3 OCA], [https://pdbe.org/5tc3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tc3 RCSB], [https://www.ebi.ac.uk/pdbsum/5tc3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tc3 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tc3 OCA], [http://pdbe.org/5tc3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tc3 RCSB], [http://www.ebi.ac.uk/pdbsum/5tc3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tc3 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/Q756Z6_ASHGO Q756Z6_ASHGO]] Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.[HAMAP-Rule:MF_03156]  
[https://www.uniprot.org/uniprot/Q756Z6_ASHGO Q756Z6_ASHGO] Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.[HAMAP-Rule:MF_03156]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Inosine-5'-monophosphate dehydrogenase (IMPDH) is an essential enzyme for nucleotide metabolism and cell proliferation. Despite IMPDH is the target of drugs with antiviral, immunosuppressive and antitumor activities, its physiological mechanisms of regulation remain largely unknown. Using the enzyme from the industrial fungus Ashbya gossypii, we demonstrate that the binding of adenine and guanine nucleotides to the canonical nucleotide binding sites of the regulatory Bateman domain induces different enzyme conformations with significantly distinct catalytic activities. Thereby, the comparison of their high-resolution structures defines the mechanistic and structural details of a nucleotide-controlled conformational switch that allosterically modulates the catalytic activity of eukaryotic IMPDHs. Remarkably, retinopathy-associated mutations lie within the mechanical hinges of the conformational change, highlighting its physiological relevance. Our results expand the mechanistic repertoire of Bateman domains and pave the road to new approaches targeting IMPDHs.


A nucleotide-controlled conformational switch modulates the activity of eukaryotic IMP dehydrogenases.,Buey RM, Fernandez-Justel D, Marcos-Alcalde I, Winter G, Gomez-Puertas P, de Pereda JM, Luis Revuelta J Sci Rep. 2017 Jun 1;7(1):2648. doi: 10.1038/s41598-017-02805-x. PMID:28572600<ref>PMID:28572600</ref>
==See Also==
 
*[[Inosine monophosphate dehydrogenase 3D structures|Inosine monophosphate dehydrogenase 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5tc3" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Ashgo]]
[[Category: Eremothecium gossypii ATCC 10895]]
[[Category: IMP dehydrogenase]]
[[Category: Large Structures]]
[[Category: Buey, R M]]
[[Category: Buey RM]]
[[Category: Fernandez-Justel, D]]
[[Category: Fernandez-Justel D]]
[[Category: Pereda, J M.de]]
[[Category: Revuelta JL]]
[[Category: Revuelta, J L]]
[[Category: De Pereda JM]]
[[Category: Allosteric modulator]]
[[Category: Ashbya gossypii]]
[[Category: Imp dehydrogenase]]
[[Category: Oxidoreductase]]
[[Category: Purine nucleotide]]
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