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<StructureSection load='2dzn' size='340' side='right'caption='[[2dzn]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='2dzn' size='340' side='right'caption='[[2dzn]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2dzn]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DZN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DZN FirstGlance]. <br>
<table><tr><td colspan='2'>[[2dzn]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DZN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DZN FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2dzo|2dzo]]</div></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dzn OCA], [https://pdbe.org/2dzn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dzn RCSB], [https://www.ebi.ac.uk/pdbsum/2dzn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dzn ProSAT], [https://www.topsan.org/Proteins/RSGI/2dzn TOPSAN]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dzn OCA], [https://pdbe.org/2dzn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dzn RCSB], [https://www.ebi.ac.uk/pdbsum/2dzn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dzn ProSAT], [https://www.topsan.org/Proteins/RSGI/2dzn TOPSAN]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/PSD10_YEAST PSD10_YEAST]] Acts as a chaperone during the assembly of the 26S proteasome, specifically of the 19S regulatory complex (RC) and appears to have an overlapping role with RPN14.<ref>PMID:19412159</ref> <ref>PMID:19446322</ref> <ref>PMID:9714768</ref> [[https://www.uniprot.org/uniprot/PRS6B_YEAST PRS6B_YEAST]] The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity).
[https://www.uniprot.org/uniprot/PSD10_YEAST PSD10_YEAST] Acts as a chaperone during the assembly of the 26S proteasome, specifically of the 19S regulatory complex (RC) and appears to have an overlapping role with RPN14.<ref>PMID:19412159</ref> <ref>PMID:19446322</ref> <ref>PMID:9714768</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2dzn ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2dzn ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The 26S proteasome-dependent protein degradation is an evolutionarily conserved process. The mammalian oncoprotein gankyrin, which associates with S6 of the proteasome, facilitates the degradation of pRb, and thus possibly acts as a bridging factor between the proteasome and its substrates. However, the mechanism of the proteasome-dependent protein degradation in yeast is poorly understood. Here, we report the tertiary structure of the complex between Nas6 and a C-terminal domain of Rpt3, which are the yeast orthologues of gankyrin and S6, respectively. The concave region of Nas6 bound to the alpha-helical domain of Rpt3. The stable interaction between Nas6 and Rpt3 was mediated by intermolecular interactions composed of complementary charged patches. The recognition of Rpt3 by Nas6 in the crystal suggests that Nas6 is indeed a subunit of the 26S proteasome. These results provide a structural basis for the association between Nas6 and the heterohexameric ATPase ring of the proteasome through Rpt3.
Structural basis for the recognition between the regulatory particles Nas6 and Rpt3 of the yeast 26S proteasome.,Nakamura Y, Umehara T, Tanaka A, Horikoshi M, Padmanabhan B, Yokoyama S Biochem Biophys Res Commun. 2007 Aug 3;359(3):503-9. Epub 2007 May 29. PMID:17555716<ref>PMID:17555716</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2dzn" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Atcc 18824]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Padmanabhan, B]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Structural genomic]]
[[Category: Padmanabhan B]]
[[Category: Yokoyama, S]]
[[Category: Yokoyama S]]
[[Category: A-helical domain]]
[[Category: Ankyrin repeat]]
[[Category: National project on protein structural and functional analyse]]
[[Category: Nppsfa]]
[[Category: Protein binding]]
[[Category: Rsgi]]

Latest revision as of 13:48, 13 March 2024

Crystal structure analysis of yeast Nas6p complexed with the proteasome subunit, rpt3

2dzn, resolution 2.20Å

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