3a5i: Difference between revisions

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<StructureSection load='3a5i' size='340' side='right'caption='[[3a5i]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
<StructureSection load='3a5i' size='340' side='right'caption='[[3a5i]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3a5i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A5I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A5I FirstGlance]. <br>
<table><tr><td colspan='2'>[[3a5i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A5I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A5I FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">flhA, STM1913 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a5i OCA], [https://pdbe.org/3a5i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a5i RCSB], [https://www.ebi.ac.uk/pdbsum/3a5i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a5i ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a5i OCA], [https://pdbe.org/3a5i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a5i RCSB], [https://www.ebi.ac.uk/pdbsum/3a5i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a5i ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/FLHA_SALTY FLHA_SALTY]] Required for formation of the rod structure of the flagellar apparatus. Together with FliI and FliH, may constitute the export apparatus of flagellin.  
[https://www.uniprot.org/uniprot/FLHA_SALTY FLHA_SALTY] Required for formation of the rod structure of the flagellar apparatus. Together with FliI and FliH, may constitute the export apparatus of flagellin.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3a5i ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3a5i ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
FlhA is the largest integral membrane component of the flagellar type III protein export apparatus of Salmonella and is composed of an N-terminal transmembrane domain (FlhA(TM)) and a C-terminal cytoplasmic domain (FlhA(C)). FlhA(C) is thought to form a platform of the export gate for the soluble components to bind to for efficient delivery of export substrates to the gate. Here, we report a structure of FlhA(C) at 2.8 A resolution. FlhA(C) consists of four subdomains (A(C)D1, A(C)D2, A(C)D3 and A(C)D4) and a linker connecting FlhA(C) to FlhA(TM). The sites of temperature-sensitive (ts) mutations that impair protein export are distributed to all four domains, with half of them at subdomain interfaces. Analyses of the ts mutations and four suppressor mutations to the G368C ts mutation suggested that FlhA(C) changes its conformation for its function. Molecular dynamics simulation demonstrated an open-close motion with a 5-10 ns oscillation in the distance between A(C)D2 and A(C)D4. These results along with further mutation analyses suggest that a dynamic domain motion of FlhA(C) is essential for protein export.
Structure of the cytoplasmic domain of FlhA and implication for flagellar type III protein export.,Saijo-Hamano Y, Imada K, Minamino T, Kihara M, Shimada M, Kitao A, Namba K Mol Microbiol. 2010 Apr;76(1):260-8. Epub 2010 Feb 28. PMID:20199603<ref>PMID:20199603</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3a5i" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Flagellar protein 3D structures|Flagellar protein 3D structures]]
*[[Flagellar protein 3D structures|Flagellar protein 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bacillus typhimurium loeffler 1892]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Imada, K]]
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
[[Category: Namba, K]]
[[Category: Imada K]]
[[Category: Saijo-Hamano, Y]]
[[Category: Namba K]]
[[Category: Shimada, M]]
[[Category: Saijo-Hamano Y]]
[[Category: Bacterial flagellum biogenesis]]
[[Category: Shimada M]]
[[Category: Bacterial flagellum protein export]]
[[Category: Cell inner membrane]]
[[Category: Cell membrane]]
[[Category: Four domain]]
[[Category: Membrane]]
[[Category: Protein transport]]
[[Category: Thioredoxin-like fold]]
[[Category: Transmembrane]]
[[Category: Transport]]
Retrieved from "https://proteopedia.org/3a5i"