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<StructureSection load='3bpq' size='340' side='right'caption='[[3bpq]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='3bpq' size='340' side='right'caption='[[3bpq]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3bpq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Metja Metja]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BPQ FirstGlance]. <br>
<table><tr><td colspan='2'>[[3bpq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii_DSM_2661 Methanocaldococcus jannaschii DSM 2661]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BPQ FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">relB3, relB, MJ1103.1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 METJA]), MJ1103, relE, relE3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 METJA])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bpq OCA], [https://pdbe.org/3bpq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bpq RCSB], [https://www.ebi.ac.uk/pdbsum/3bpq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bpq ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bpq OCA], [https://pdbe.org/3bpq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bpq RCSB], [https://www.ebi.ac.uk/pdbsum/3bpq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bpq ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/RELB3_METJA RELB3_METJA]] Antitoxin component of a toxin-antitoxin (TA) module. Probably neutralizes the toxic activity of cognate toxin RelE (By similarity).<ref>PMID:15718296</ref> [[https://www.uniprot.org/uniprot/RELE3_METJA RELE3_METJA]] Toxic component of a toxin-antitoxin (TA) module. Has RNase activity (By similarity). Is very toxic upon expression in E.coli. Its toxic activity is probably neutralized by the cognate antitoxin RelB3.<ref>PMID:15718296</ref> 
[https://www.uniprot.org/uniprot/RELB3_METJA RELB3_METJA] Antitoxin component of a toxin-antitoxin (TA) module. Probably neutralizes the toxic activity of cognate toxin RelE (By similarity).<ref>PMID:15718296</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bpq ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bpq ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Here we present the crystal structure of the Methanococcus jannaschii RelE-RelB (RelBE) toxin-antitoxin (TA) protein complex determined by the MIRAS (multiple isomorphous replacement with anomalous signal) method. The genes encoding this TA system are located in the chromosome of this archaeon and involved in stress response. RelE acts as an endoribonuclease that cleaves mRNA on the ribosome, and we compare the RelBE complex to the known structures of other TA systems belonging to this group and to endoribonucleases. M. jannaschii RelBE forms a heterotetramer with the antitoxin in the centre of the complex, a configuration that differs vastly from the heterotetramer structure of the previously published RelBE from another archaeon, Pyrococcus horikoshii. The long N-terminal alpha-helix of the tightly bound M. jannaschii antitoxin RelB covers the presumed active site of the toxin RelE that is formed by a central beta-sheet, a loop on one side and a C-terminal alpha-helix on the other side. The active site of the M. jannaschii toxin RelE harbours positive charges that are thought to neutralize the negative charges of the substrate mRNA, including Arg62 that was changed to Ser62 by the Escherichia coli expression system, thereby leading to inactive toxin RelE. Comparative studies suggest that Asp43 and His79 are also involved in the activity of the toxin.
Crystal structure of the antitoxin-toxin protein complex RelB-RelE from Methanococcus jannaschii.,Francuski D, Saenger W J Mol Biol. 2009 Nov 6;393(4):898-908. Epub 2009 Aug 25. PMID:19712680<ref>PMID:19712680</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3bpq" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Metja]]
[[Category: Methanocaldococcus jannaschii DSM 2661]]
[[Category: Francuski, D]]
[[Category: Francuski D]]
[[Category: Saenger, W]]
[[Category: Saenger W]]
[[Category: Protein toxin-antitoxin complex]]
[[Category: Toxin]]

Latest revision as of 14:03, 13 March 2024

Crystal Structure of RelB-RelE antitoxin-toxin complex from Methanococcus jannaschii

3bpq, resolution 2.20Å

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