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| <StructureSection load='5vy6' size='340' side='right'caption='[[5vy6]], [[Resolution|resolution]] 3.06Å' scene=''> | | <StructureSection load='5vy6' size='340' side='right'caption='[[5vy6]], [[Resolution|resolution]] 3.06Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[5vy6]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VY6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VY6 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[5vy6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VY6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VY6 FirstGlance]. <br> |
| </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5vy7|5vy7]]</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.064Å</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vy6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vy6 OCA], [http://pdbe.org/5vy6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vy6 RCSB], [http://www.ebi.ac.uk/pdbsum/5vy6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vy6 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vy6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vy6 OCA], [https://pdbe.org/5vy6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vy6 RCSB], [https://www.ebi.ac.uk/pdbsum/5vy6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vy6 ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| The foundational goal of structural DNA nanotechnology-the field that uses oligonucleotides as a molecular building block for the programmable self-assembly of nanostructured systems-was to use DNA to construct three-dimensional (3D) lattices for solving macromolecular structures. The programmable nature of DNA makes it an ideal system for rationally constructing self-assembled crystals and immobilizing guest molecules in a repeating 3D array through their specific stereospatial interactions with the scaffold. In this work, we have extended a previously described motif (4 x 5) by expanding the structure to a system that links four double-helical layers; we use a central weaving oligonucleotide containing a sequence of four six-base repeats (4 x 6), forming a matrix of layers that are organized and dictated by a series of Holliday junctions. In addition, we have assembled mirror image crystals (l-DNA) with the identical sequence that are completely resistant to nucleases. Bromine and selenium derivatives were obtained for the l- and d-DNA forms, respectively, allowing phase determination for both forms and solution of the resulting structures to 3.0 and 3.05 A resolution. Both right- and left-handed forms crystallized in the trigonal space groups with mirror image 3-fold helical screw axes P32 and P31 for each motif, respectively. The structures reveal a highly organized array of discrete and well-defined cavities that are suitable for hosting guest molecules and allow us to dictate a priori the assembly of guest-DNA conjugates with a specified crystalline hand.
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| Tuning the Cavity Size and Chirality of Self-Assembling 3D DNA Crystals.,Simmons CR, Zhang F, MacCulloch T, Fahmi N, Stephanopoulos N, Liu Y, Seeman NC, Yan H J Am Chem Soc. 2017 Aug 2. doi: 10.1021/jacs.7b06485. PMID:28731332<ref>PMID:28731332</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 5vy6" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Simmons, C R]] | | [[Category: Synthetic construct]] |
| [[Category: Yan, H]] | | [[Category: Simmons CR]] |
| [[Category: Dna]] | | [[Category: Yan H]] |
| [[Category: Dna nanotechnology]]
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| [[Category: Dna structure]]
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| [[Category: Self-assembly]]
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