6b85: Difference between revisions

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<StructureSection load='6b85' size='340' side='right'caption='[[6b85]], [[Resolution|resolution]] 3.89&Aring;' scene=''>
<StructureSection load='6b85' size='340' side='right'caption='[[6b85]], [[Resolution|resolution]] 3.89&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6b85]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B85 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B85 FirstGlance]. <br>
<table><tr><td colspan='2'>[[6b85]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B85 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B85 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b85 OCA], [http://pdbe.org/6b85 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b85 RCSB], [http://www.ebi.ac.uk/pdbsum/6b85 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b85 ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.889&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b85 OCA], [https://pdbe.org/6b85 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b85 RCSB], [https://www.ebi.ac.uk/pdbsum/6b85 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b85 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The computational design of transmembrane proteins with more than one membrane-spanning region remains a major challenge. We report the design of transmembrane monomers, homodimers, trimers, and tetramers with 76 to 215 residue subunits containing two to four membrane-spanning regions and up to 860 total residues that adopt the target oligomerization state in detergent solution. The designed proteins localize to the plasma membrane in bacteria and in mammalian cells, and magnetic tweezer unfolding experiments in the membrane indicate that they are very stable. Crystal structures of the designed dimer and tetramer-a rocket-shaped structure with a wide cytoplasmic base that funnels into eight transmembrane helices-are very close to the design models. Our results pave the way for the design of multispan membrane proteins with new functions.
Accurate computational design of multipass transmembrane proteins.,Lu P, Min D, DiMaio F, Wei KY, Vahey MD, Boyken SE, Chen Z, Fallas JA, Ueda G, Sheffler W, Mulligan VK, Xu W, Bowie JU, Baker D Science. 2018 Mar 2;359(6379):1042-1046. doi: 10.1126/science.aaq1739. PMID:29496880<ref>PMID:29496880</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6b85" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Synthetic construct sequences]]
[[Category: Synthetic construct]]
[[Category: Baker, D]]
[[Category: Baker D]]
[[Category: Bowie, J]]
[[Category: Bowie J]]
[[Category: DiMaio, F]]
[[Category: DiMaio F]]
[[Category: Lu, P]]
[[Category: Lu P]]
[[Category: Min, D]]
[[Category: Min D]]
[[Category: Wei, K Y]]
[[Category: Wei KY]]
[[Category: De novo design]]
[[Category: Helical bundle]]
[[Category: Helical repeat protein]]
[[Category: Membrane protein]]
[[Category: Multipass transmembrane protein]]
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