6cs8: Difference between revisions

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<StructureSection load='6cs8' size='340' side='right'caption='[[6cs8]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
<StructureSection load='6cs8' size='340' side='right'caption='[[6cs8]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6cs8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CS8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6CS8 FirstGlance]. <br>
<table><tr><td colspan='2'>[[6cs8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CS8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CS8 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F9Y:1H-indole-6-carbonitrile'>F9Y</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.749&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6cs8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cs8 OCA], [http://pdbe.org/6cs8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cs8 RCSB], [http://www.ebi.ac.uk/pdbsum/6cs8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cs8 ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F9Y:1H-indole-6-carbonitrile'>F9Y</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cs8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cs8 OCA], [https://pdbe.org/6cs8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cs8 RCSB], [https://www.ebi.ac.uk/pdbsum/6cs8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cs8 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/FTSY_ECOLI FTSY_ECOLI]] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC). Interaction with SRP-RNC leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components.<ref>PMID:8194520</ref> <ref>PMID:9305630</ref> <ref>PMID:11735405</ref> <ref>PMID:11741850</ref> <ref>PMID:15148364</ref> <ref>PMID:17682051</ref> 
[https://www.uniprot.org/uniprot/FTSY_ECOLI FTSY_ECOLI] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC). Interaction with SRP-RNC leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components.<ref>PMID:8194520</ref> <ref>PMID:9305630</ref> <ref>PMID:11735405</ref> <ref>PMID:11741850</ref> <ref>PMID:15148364</ref> <ref>PMID:17682051</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Given the increasing incidence of antibiotic resistance, antibiotics that employ new strategies are urgently needed. Bacterial survival is dependent on proper function of the signal recognition particle (SRP) and its receptor (FtsY). A unique set of interactions in FtsY:SRP-RNA represents a promising candidate for new antibiotic development as no antibiotic targets this complex and these interactions are functionally replaced by protein:protein interactions in eukaryotes. We used a Fragment Based Drug Design (FBDD) approach to search for new compounds that can bind FtsY, and have identified three lead fragments. In vitro and in vivo analyses have shown that despite a high micromolar binding affinity, one fragment has some antimicrobial properties. X-ray structures of E. coli FtsY:fragments reveal the fragments bind in the targeted RNA interaction site. Our results show that FBDD is a suitable approach for targeting FtsY:SRP-RNA for antibiotic development and opens the possibility of targeting protein:RNA interactions in general.
 
Discovery of fragments that target key interactions in the signal recognition particle (SRP) as potential leads for a new class of antibiotics.,Faoro C, Wilkinson-White L, Kwan AH, Ataide SF PLoS One. 2018 Jul 25;13(7):e0200387. doi: 10.1371/journal.pone.0200387., eCollection 2018. PMID:30044812<ref>PMID:30044812</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6cs8" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Signal recognition particle receptor|Signal recognition particle receptor]]
*[[Signal recognition particle receptor 3D structures|Signal recognition particle receptor 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Ataide, S F]]
[[Category: Ataide SF]]
[[Category: Faoro, C]]
[[Category: Faoro C]]
[[Category: Cotranslational delivery srp]]
[[Category: Protein transport]]

Latest revision as of 14:30, 13 March 2024

High resolution crystal structure of FtsY-NG domain of E. coli

6cs8, resolution 1.75Å

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