6e13: Difference between revisions

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<StructureSection load='6e13' size='340' side='right'caption='[[6e13]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
<StructureSection load='6e13' size='340' side='right'caption='[[6e13]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6e13]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Psepk Psepk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E13 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E13 FirstGlance]. <br>
<table><tr><td colspan='2'>[[6e13]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida_KT2440 Pseudomonas putida KT2440]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E13 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6E13 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HKS:3-{[(2S)-2-amino-2-carboxyethyl]sulfanyl}-5-hydroxy-L-tyrosine'>HKS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.349&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pqqB, PP_0379 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160488 PSEPK])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HKS:3-{[(2S)-2-amino-2-carboxyethyl]sulfanyl}-5-hydroxy-L-tyrosine'>HKS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e13 OCA], [http://pdbe.org/6e13 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e13 RCSB], [http://www.ebi.ac.uk/pdbsum/6e13 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e13 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6e13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e13 OCA], [https://pdbe.org/6e13 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6e13 RCSB], [https://www.ebi.ac.uk/pdbsum/6e13 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6e13 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/PQQB_PSEPK PQQB_PSEPK]] May be involved in the transport of PQQ or its precursor to the periplasm (By similarity).  
[https://www.uniprot.org/uniprot/PQQB_PSEPK PQQB_PSEPK] May be involved in the transport of PQQ or its precursor to the periplasm (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Understanding the biosynthesis of cofactors is fundamental to the life sciences, yet to date a few important pathways remain unresolved. One example is the redox cofactor pyrroloquinoline quinone (PQQ), which is critical for C1 metabolism in many microorganisms, a disproportionate number of which are opportunistic human pathogens. While the initial and final steps of PQQ biosynthesis, involving PqqD/E and PqqC, have been elucidated, the precise nature and order of the remaining transformations in the pathway are unknown. Here we show evidence that the remaining essential biosynthetic enzyme PqqB is an iron-dependent hydroxylase catalyzing oxygen-insertion reactions that are proposed to produce the quinone moiety of the mature PQQ cofactor. The demonstrated reactions of PqqB are unprecedented within the metallo beta-lactamase protein family and expand the catalytic repertoire of nonheme iron hydroxylases. These new findings also generate a nearly complete description of the PQQ biosynthetic pathway.
 
Discovery of Hydroxylase Activity for PqqB Provides a Missing Link in the Pyrroloquinoline Quinone Biosynthetic Pathway.,Koehn EM, Latham JA, Armand T, Evans RL 3rd, Tu X, Wilmot CM, Iavarone AT, Klinman JP J Am Chem Soc. 2019 Mar 13;141(10):4398-4405. doi: 10.1021/jacs.8b13453. Epub, 2019 Feb 27. PMID:30811189<ref>PMID:30811189</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6e13" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Psepk]]
[[Category: Pseudomonas putida KT2440]]
[[Category: III, R L.Evans]]
[[Category: Evans III RL]]
[[Category: Wilmot, C M]]
[[Category: Wilmot CM]]
[[Category: Hydroxylase]]
[[Category: Iron-dependent]]
[[Category: Metallo beta-lactamase]]
[[Category: Oxidoreductase]]

Latest revision as of 14:38, 13 March 2024

Pseudomonas putida PqqB with a non-physiological zinc at the active site binds the substrate mimic, 5-cysteinyl-3,4-dihydroxyphenylalanine (5-Cys-DOPA), non-specifically but supports the proposed function of the enzyme in pyrroloquinoline quinone biosynthesis.

6e13, resolution 2.35Å

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