6n07: Difference between revisions

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<SX load='6n07' size='340' side='right' viewer='molstar' caption='[[6n07]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
<SX load='6n07' size='340' side='right' viewer='molstar' caption='[[6n07]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6n07]] is a 42 chain structure with sequence from [http://en.wikipedia.org/wiki/Halo1 Halo1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N07 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6N07 FirstGlance]. <br>
<table><tr><td colspan='2'>[[6n07]] is a 42 chain structure with sequence from [https://en.wikipedia.org/wiki/Haliangium_ochraceum_DSM_14365 Haliangium ochraceum DSM 14365]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N07 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N07 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6mzx|6mzx]], [[6mzy|6mzy]], [[6n06|6n06]], [[6mzu|6mzu]], [[6mzv|6mzv]], [[6n0f|6n0f]], [[6n09|6n09]], [[6n0g|6n0g]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Hoch_5816 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=502025 HALO1]), Hoch_5815 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=502025 HALO1])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n07 OCA], [https://pdbe.org/6n07 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n07 RCSB], [https://www.ebi.ac.uk/pdbsum/6n07 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n07 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6n07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n07 OCA], [http://pdbe.org/6n07 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n07 RCSB], [http://www.ebi.ac.uk/pdbsum/6n07 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n07 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/BMCT2_HALO1 BMCT2_HALO1] A minor component of the bacterial microcompartment (BMC) shell. Expression of 5 proteins in E.coli (BMC-H (Hoch_5815), BMC-P (Hoch_5814), and 3 BMC-T (Hoch_5812, Hoch_5816, Hoch_3341)) forms 40 nm artificial BMCs with a molecular mass of 6.5 MDa. One of 2 stacked pseudohexamers in the BMC. There are 20 BMC-T pseudohexamers per BMC, composed of mixed BMC-T1, BMC-T2 and BMC-T3. The shell facets are 20-30 Angstroms thick, with 1 of the stacked BMC-T trimers protruding to the exterior (PubMed:28642439, PubMed:30833088). The stacked trimers may serve as conduits to allow chemical flux across the protein shell, gated by Arg-70 which contacts Glu-69 in an adjacent subunit; they are flexible enough to play a role in accommodating variations in shell assembly (Probable).<ref>PMID:28642439</ref> <ref>PMID:30833088</ref> <ref>PMID:30833088</ref>  
Bacterial microcompartments (BMCs) are composed of an enzymatic core encapsulated by a selectively permeable protein shell that enhances catalytic efficiency. Many pathogenic bacteria derive competitive advantages from their BMC-based catabolism, implicating BMCs as drug targets. BMC shells are of interest for bioengineering due to their diverse and selective permeability properties and because they self-assemble. A complete understanding of shell composition and organization is a prerequisite for biotechnological applications. Here, we report the cryoelectron microscopy structure of a BMC shell at 3.0-A resolution, using an image-processing strategy that allowed us to determine the previously uncharacterized structural details of the interactions formed by the BMC-T(S) and BMC-T(D) shell subunits in the context of the assembled shell. We found unexpected structural plasticity among these interactions, resulting in distinct shell populations assembled from varying numbers of the BMC-T(S) and BMC-T(D) subunits. We discuss the implications of these findings on shell assembly and function.
 
The Plasticity of Molecular Interactions Governs Bacterial Microcompartment Shell Assembly.,Greber BJ, Sutter M, Kerfeld CA Structure. 2019 Feb 12. pii: S0969-2126(19)30017-6. doi:, 10.1016/j.str.2019.01.017. PMID:30833088<ref>PMID:30833088</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6n07" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</SX>
</SX>
[[Category: Halo1]]
[[Category: Haliangium ochraceum DSM 14365]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Greber, B J]]
[[Category: Greber BJ]]
[[Category: Kerfeld, C A]]
[[Category: Kerfeld CA]]
[[Category: Sutter, M]]
[[Category: Sutter M]]
[[Category: Bmc fold]]
[[Category: Compartmentalization]]
[[Category: Microcompartment]]
[[Category: Shell]]
[[Category: Structural protein]]

Latest revision as of 14:46, 13 March 2024

Structure of the HO BMC shell: BMC-TD focused map, open inner pore, compacted shell

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