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| <StructureSection load='6o43' size='340' side='right'caption='[[6o43]], [[Resolution|resolution]] 2.08Å' scene=''> | | <StructureSection load='6o43' size='340' side='right'caption='[[6o43]], [[Resolution|resolution]] 2.08Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6o43]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_phage_p68 Staphylococcus aureus phage p68]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O43 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O43 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[6o43]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_phage_P68 Staphylococcus phage P68]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O43 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6O43 FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=I3C:5-AMINO-2,4,6-TRIIODOBENZENE-1,3-DICARBOXYLIC+ACID'>I3C</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.0822299Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Orf11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=204090 Staphylococcus aureus phage P68])</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=I3C:5-AMINO-2,4,6-TRIIODOBENZENE-1,3-DICARBOXYLIC+ACID'>I3C</scene></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6o43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o43 OCA], [http://pdbe.org/6o43 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o43 RCSB], [http://www.ebi.ac.uk/pdbsum/6o43 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o43 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6o43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o43 OCA], [https://pdbe.org/6o43 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6o43 RCSB], [https://www.ebi.ac.uk/pdbsum/6o43 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6o43 ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/Q859J2_9CAUD Q859J2_9CAUD] |
| Two commonly encountered bottlenecks in the structure determination of a protein by X-ray crystallography are screening for conditions that give high-quality crystals and, in the case of novel structures, finding derivatization conditions for experimental phasing. In this study, the phasing molecule 5-amino-2,4,6-triiodoisophthalic acid (I3C) was added to a random microseed matrix screen to generate high-quality crystals derivatized with I3C in a single optimization experiment. I3C, often referred to as the magic triangle, contains an aromatic ring scaffold with three bound I atoms. This approach was applied to efficiently phase the structures of hen egg-white lysozyme and the N-terminal domain of the Orf11 protein from Staphylococcus phage P68 (Orf11 NTD) using SAD phasing. The structure of Orf11 NTD suggests that it may play a role as a virion-associated lysin or endolysin.
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| Combining random microseed matrix screening and the magic triangle for the efficient structure solution of a potential lysin from bacteriophage P68.,Truong JQ, Panjikar S, Shearwin-Whyatt L, Bruning JB, Shearwin KE Acta Crystallogr D Struct Biol. 2019 Jul 1;75(Pt 7):670-681. doi:, 10.1107/S2059798319009008. Epub 2019 Jul 2. PMID:31282476<ref>PMID:31282476</ref>
| | ==See Also== |
| | | *[[Lysin 3D structures|Lysin 3D structures]] |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
| |
| <div class="pdbe-citations 6o43" style="background-color:#fffaf0;"></div>
| |
| == References == | |
| <references/>
| |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Staphylococcus aureus phage p68]] | | [[Category: Staphylococcus phage P68]] |
| [[Category: Truong, J Q]] | | [[Category: Truong JQ]] |
| [[Category: Antimicrobial protein]]
| |
| [[Category: Bacteriophage protein]]
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| [[Category: Lysin]]
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