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| <StructureSection load='6ood' size='340' side='right'caption='[[6ood]], [[Resolution|resolution]] 1.50Å' scene=''> | | <StructureSection load='6ood' size='340' side='right'caption='[[6ood]], [[Resolution|resolution]] 1.50Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6ood]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Garden_pea Garden pea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OOD OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OOD FirstGlance]. <br> | | <table><tr><td colspan='2'>[[6ood]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OOD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OOD FirstGlance]. <br> |
| </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ood FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ood OCA], [http://pdbe.org/6ood PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ood RCSB], [http://www.ebi.ac.uk/pdbsum/6ood PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ood ProSAT]</span></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ood FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ood OCA], [https://pdbe.org/6ood PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ood RCSB], [https://www.ebi.ac.uk/pdbsum/6ood PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ood ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| The biochemical activities of dirigent proteins (DPs) give rise to distinct complex classes of plant phenolics. DPs apparently began to emerge during the aquatic-to-land transition, with phylogenetic analyses revealing the presence of numerous DP subfamilies in the plant kingdom. The vast majority (>95%) of DPs in these large multigene families still await discovery of their biochemical functions. Here, we elucidated the 3D structures of two pterocarpan-forming proteins with dirigent-like domains. Both proteins stereospecifically convert distinct diastereomeric chiral isoflavonoid precursors to the chiral pterocarpans, (-)- and (+)-medicarpin, respectively. Their 3D structures enabled comparisons with stereoselective lignan- and aromatic terpenoid-forming DP orthologs. Each protein provides entry into diverse plant natural products classes, and our experiments suggest a common biochemical mechanism in binding and stabilizing distinct plant phenol-derived mono- and bis-quinone methide intermediates during different C-C and C-O bond-forming processes. These observations provide key insights into both their appearance and functional diversification of DPs during land plant evolution/adaptation. The proposed biochemical mechanisms based on our findings provide important clues to how additional physiological roles for DPs and proteins harboring dirigent-like domains can now be rationally and systematically identified.
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| Pterocarpan synthase (PTS) structures suggest a common quinone methide-stabilizing function in dirigent proteins and proteins with dirigent-like domains.,Meng Q, Moinuddin SGA, Kim SJ, Bedgar DL, Costa MA, Thomas DG, Young RP, Smith CA, Cort JR, Davin LB, Lewis NG J Biol Chem. 2020 Aug 14;295(33):11584-11601. doi: 10.1074/jbc.RA120.012444. Epub, 2020 Jun 21. PMID:32565424<ref>PMID:32565424</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 6ood" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Garden pea]]
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| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Smith, C A]] | | [[Category: Pisum sativum]] |
| [[Category: Apo-form]] | | [[Category: Smith CA]] |
| [[Category: Dirigent protein]]
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| [[Category: Plant protein]]
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